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Rabbit Anti-CD27 antibody (bsm-54318R)

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说明书: 50ul  100ul  
50ul/1580.00元
100ul/2680.00元
大包装/询价

产品编号 bsm-54318R
英文名称 CD27
中文名称 CD27重组兔单克隆抗体
别    名 CD 27; CD27 antigen; CD27 molecule; CD27_HUMAN; CD27L receptor; LPFS2; MGC20393; OTTHUMP00000238557; S152; T cell activation antigen CD27; T cell antivation antigen S152; T-cell activation antigen CD27; T14; TNFRSF 7; TNFRSF7; TNFSF7; Tp 55; Tp55; Tumor necrosis factor receptor superfamily member 7.  
研究领域 肿瘤  免疫学  干细胞  淋巴细胞  t-淋巴细胞  细胞因子  
抗体来源 Rabbit
克隆类型 Monoclonal
克 隆 号 3C3
交叉反应 Mouse,  (predicted: Human, Rat, )
产品应用 WB=1:500-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 55kDa
细胞定位 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD27: 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]

Function:
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Subunit:
Homodimer. Interacts with SIVA1 and TRAF2.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Found in most T-lymphocytes.

Post-translational modifications:
Phosphorylated.
O-glycosylated with core 1 or possibly core 8 glycans.

Similarity:
Contains 3 TNFR-Cys repeats.

SWISS:
P26842

Gene ID:
939

Database links:

Entrez Gene: 939 Human

Entrez Gene: 21940 Mouse

Entrez Gene: 500318 Rat

Omim: 186711 Human

SwissProt: P26842 Human

SwissProt: P41272 Mouse

Unigene: 355307 Human

Unigene: 367714 Mouse

Unigene: 134200 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug
Lane 2: Lymph node (Mouse) Lysate at 40 ug
Lane 3: Thymus (Mouse) Lysate at 40 ug
Lane 4: Plasma (Mouse) at 40 ug
Primary: Anti- CD27 (bsm-54318R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Paraformaldehyde-fixed, paraffin embedded (mouse spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD27) Monoclonal Antibody, Unconjugated (bsm-54318R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD27) Monoclonal Antibody, Unconjugated (bsm-54318R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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