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Rabbit Anti-Integrin alpha 2 antibody (bsm-52613R)

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说明书: 50ul  100ul  
50ul/1580.00元
100ul/2680.00元
大包装/询价

产品编号 bsm-52613R
英文名称 Integrin alpha 2
中文名称 整合素α2重组兔单克隆抗体
别    名 Integrin alpha 2; CD49b antigen; BR; CD 49b; CD49 antigen like family member B; CD49b; CD49b; Collagen receptor; DX5; Glycoprotein Ia deficiency included; GP Ia; GP Ia deficiency, included; GPIa; HPA 5 included; HPA5 included; Itga2; Platelet alloantigen Br(a), included; Platelet antigen Br; Platelet glycoprotein GPIa; Platelet glycoprotein Ia; Platelet glycoprotein Ia/Iia; Platelet membrane glycoprotein Ia; Platelet receptor for collagen, deficiency of, included; Very late activation protein 2 receptor alpha 2 subunit; VLA 2 alpha chain; VLA 2; VLA 2 subunit alpha; VLA2 alpha chain; ITA2_HUMAN.  
研究领域 肿瘤  细胞生物  细胞粘附分子  
抗体来源 Rabbit
克隆类型 Monoclonal
克 隆 号 14D7
交叉反应 Human, Mouse, Rat, 
产品应用 WB=1:500-2000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:100-500 IF=1:50-200 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 127kDa
细胞定位 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 Recombinant human Integrin alpha 2: 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. They are responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells.

Function:
Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.

Subunit:
Heterodimer of an alpha and a beta subunit. Alpha-2 associates with beta-1. Interacts with HPS5 and RAB21. Binds to human echoviruses 1 and 8 capsid proteins and acts as a receptor for these viruses. Interacts (via ITAG2 I-domain) with rotavirus A VP4; this interaction only occurs in rotavirus integrin-dependent strains, for which ITGA2 acts as a receptor.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Similarity:
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.

SWISS:
P17301

Gene ID:
3673

Database links:

Entrez Gene: 3673 Human

Entrez Gene: 16398 Mouse

Omim: 192974 Human

SwissProt: P17301 Human

SwissProt: Q62469 Mouse

Unigene: 482077 Human

Unigene: 5007 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample:
Lane 1: Kidney (Mouse) Lysate at 40 ug
Lane 2: Large intestine (Mouse) Lysate at 40 ug
Lane 3: Stomach (Mouse) Lysate at 40 ug
Lane 4: Skin (Mouse) Lysate at 40 ug
Lane 5: Kidney (Rat) Lysate at 40 ug
Lane 6: Large intestine (Rat) Lysate at 40 ug
Lane 7: Stomach (Rat) Lysate at 40 ug
Lane 8: Skin (Rat) Lysate at 40 ug
Lane 9: HT1080 (Human) Cell Lysate at 30 ug
Lane 10: MCF-7 (Human) Cell Lysate at 30 ug
Lane 11: Huvec (Human) Cell Lysate at 30 ug
Primary: Anti-Integrin alpha 2 (bsm-52613R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140 kD
Observed band size: 140 kD
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Integrin alpha 2) Monoclonal Antibody, Unconjugated (bsm-52613R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Integrin alpha 2) Monoclonal Antibody, Unconjugated (bsm-52613R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human skin carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Integrin alpha 2) Monoclonal Antibody, Unconjugated (bsm-52613R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Integrin alpha 2) Monoclonal Antibody, Unconjugated (bsm-52613R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Integrin alpha 2) Monoclonal Antibody, Unconjugated (bsm-52613R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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