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说明书: 50ul  100ul  
50ul/1150.00元
100ul/1880.00元
大包装/询价

产品编号 bsm-51575M
英文名称 GATA3
中文名称 GATA结合蛋白3单克隆抗体
别    名 GATA 3; GATA-3; GATA binding factor 3; GATA binding protein 3; HDR; MGC2346; MGC5199; MGC5445; Trans acting T cell specific transcription factor GATA 3; GATA3_HUMAN; Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3.  
研究领域 免疫学  发育生物学  染色质和核信号  干细胞  t-淋巴细胞  锌指蛋白  表观遗传学  
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 G3G4
交叉反应 Human, 
产品应用 WB=1:500-1000 Flow-Cyt=1:25 ICC=1:25 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 49kDa
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 Recombinant human GATA3.: 
亚    型 IgG2b,κ
纯化方法 affinity purified by Protein G
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as in erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. Both GATA-4 and GATA-6 are found in heart, pancreas and ovary; lung and liver tissues exhibit GATA-6, but not GATA-4 expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart. Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites.

Function:
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.

Subcellular Location:
Nucleus.

Tissue Specificity:
T-cells and endothelial cells.

DISEASE:
Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.

Similarity:
Contains 2 GATA-type zinc fingers.

SWISS:
P23771

Gene ID:
2625

Database links:

Entrez Gene: 2625 Human

SwissProt: P23771 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample:
Lane 1: CEM cell lysates
Lane 2: HL60 cell lysates
Lane 3: SH-SY5Y cell lysates
Primary: Anti-GATA3 (bsm-51575M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 52 kD
MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (GATA3) monoclonal Antibody, Unconjugated (bsm-51575M) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes,Alexa Fluor® 555 conjugated with Phalloidin(red) was used to stain the cell Cytoplasmic actin.
Blank control: MCF7.
Primary Antibody (green line): Mouse Anti-GATA3 antibody (bsm-51575M)
Dilution: 1:25;
Isotype Control Antibody (blue line): Mouse IgG2b Secondary Antibody : Goat anti-mouse IgG-AF488
Dilution:1:400 Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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