扫码关注公众号           扫码咨询技术支持
客服热线:400-901-9800  客服QQ:4009019800  技术答疑  技术支持  质量反馈  人才招聘  关于我们  联系我们
体育娱乐网址平台_首页
首页 > 产品中心 > 一抗 > 产品信息
Rabbit Anti-Vinculin (Loading Control) antibody (bsm-54148R)

~~~20周年感恩回馈“万能卡”~~~
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购传真:010-58129612
技术支持:techsupport@bioss.com.cn
说明书: 50ul  100ul  
50ul/1580.00元
100ul/2680.00元
大包装/询价

产品编号 bsm-54148R
英文名称 Vinculin (Loading Control)
中文名称 粘着斑蛋白(内参)重组兔单克隆抗体
别    名 CMD1W; CMH15; Epididymis luminal protein 114; HEL114; Metavinculin; MV; MVCL; OTTHUMP00000019861; OTTHUMP00000019862; VCL; VINC; VINC_HUMAN。  
产品类型 体育娱乐网址平台 
研究领域 心血管  细胞生物  信号转导  细胞粘附分子  细胞骨架  
抗体来源 Rabbit
克隆类型 Monoclonal
克 隆 号 5G7
交叉反应 Human, Mouse, Rat, 
产品应用 WB=1:500-2000 IP=1:10-50 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 124kDa
细胞定位 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 Recombinant Human Vinculin: 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Function:
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Subunit:
Exhibits self-association properties. Interacts with NRAP and SORBS1 (By similarity). Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin.

Subcellular Location:
Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Tissue Specificity:
Metavinculin is muscle-specific.

Post-translational modifications:
Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques (By similarity).
Aceylated; mainly by myristic acid but also small amount of palmitic acid (By similarity).

DISEASE:
Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) [MIM:611407]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in VCL are the cause of cardiomyopathy familial hypertrophic type 15 (CMH15) [MIM:613255]. It is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.

Similarity:
Belongs to the vinculin/alpha-catenin family.

SWISS:
P18206

Gene ID:
7414

Database links:

Entrez Gene: 7414 Human

Entrez Gene: 22330 Mouse

Entrez Gene: 305679 Rat

Omim: 193065 Human

SwissProt: P18206 Human

SwissProt: Q64727 Mouse

SwissProt: P85972 Rat

Unigene: 643896 Human

Unigene: 279361 Mouse

Unigene: 164613 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample:
Lane 1: Skeletal muscle (Mouse) Lysate at 40 ug
Lane 2: Skeletal muscle (Rat) Lysate at 40 ug
Lane 3: Bladder (Mouse) Lysate at 40 ug
Lane 4: Bladder (Rat) Lysate at 40 ug
Lane 5: U251 (Human) Cell Lysate at 30 ug
Lane 6: HUVEC (Human) Cell Lysate at 30 ug
Lane 7: A431 (Human) Cell Lysate at 30 ug
Lane 8: HepG2 (Human) Cell Lysate at 30 ug
Lane 9: Spleen (Mouse) Lysate at 40 ug
Lane 10: Spleen (Rat) Lysate at 40 ug
Lane 11: Kidney (Mouse) Lysate at 40 ug
Lane 12: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-Vinculin (bsm-54148R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 120 kD
Observed band size: 120 kD
Sample:
Lane 1: Skeletal muscle (Mouse) Lysate at 40 ug
Lane 2: Skeletal muscle (Rat) Lysate at 40 ug
Lane 3: Bladder (Mouse) Lysate at 40 ug
Lane 4: Bladder (Rat) Lysate at 40 ug
Lane 5: U251 (Human) Cell Lysate at 30 ug
Lane 6: HUVEC (Human) Cell Lysate at 30 ug
Lane 7: A431 (Human) Cell Lysate at 30 ug
Lane 8: HepG2 (Human) Cell Lysate at 30 ug
Lane 9: Spleen (Mouse) Lysate at 40 ug
Lane 10: Spleen (Rat) Lysate at 40 ug
Lane 11: Kidney (Mouse) Lysate at 40 ug
Lane 12: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-Vinculin (bsm-54148R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 120 kD
Observed band size: 120 kD
版权所有 2019-2022 体育娱乐网址平台
通过国际质量管理体系ISO9001:2008 GB/T19001-2008认证    编号:00115Q211807R1M/1100
京ICP备05066980号-1         京公网安备110107000727号