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说明书: 50ul  100ul  500ul
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产品编号 bsm-33019M
英文名称 GFP-Tag
中文名称 绿色荧光蛋白单克隆抗体
别    名 Green fluorescence protein; GFP Tag; GFPTag  
产品类型 标签抗体 
研究领域 细胞生物  海洋生物  
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 4G6
交叉反应 Recombinant protein
产品应用 WB=1:50000-500000 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 27kDa
性    状 Liquid
浓    度 1mg/ml
免 疫 原 Recombinant Green Fluorescence Protein: 
亚    型 IgG
纯化方法 affinity purified by Protein G
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

Function:
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Subunit:
Monomer.

Tissue Specificity:
Photocytes.

Post-translational modifications:
Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

Similarity:
Belongs to the GFP family.

SWISS:
P42212

Gene ID:
107331116

Database links:

 



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample:
GFP-tagged fusion protein Overexpression E.coli Lysate (Cat#: bs-33009P) at 4 ug
Primary:
Anti-GFP-Tag (bsm-33019M) at 1/1000000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 30 kD
Sample:
GFP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-33009P) at 4 ug
Primary: Anti-GST tag (bsm-33019M) at 1/5000 ~ 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
Sample:
GFP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: bs-33009P) at 2 ug
Primary: Anti-GST tag (bsm-33019M) at 1/5000 ~ 1/10000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
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