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说明书: 50ul  100ul  200ul
50ul/1138.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-20638R
英文名称 CD20
中文名称 CD20抗体
别    名 CD20_HUMAN; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; MS4A1; MS4A2; B1; CVID5; LEU-16; S7.  
研究领域 肿瘤  细胞生物  免疫学  干细胞  细胞类型标志物  淋巴细胞  b-淋巴细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse,  (predicted: Rat, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1μg /Test 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 33kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD20:101-200/297 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit.

Function:
This protein may be involved in the regulation of B-cell activation and proliferation

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed on B-cells.

Post-translational modifications:
Phosphorylated. Might be functionally regulated by protein kinase(s).

DISEASE:
Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.

Similarity:
Belongs to the MS4A family.

SWISS:
P11836

Gene ID:
931

Database links:

Entrez Gene: 931 Human

Omim: 112210 Human

SwissProt: P11836 Human

Unigene: 712553 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

B淋巴细胞标志物

CD20是B细胞特异性抗原,其表达呈细胞类型一种质膜蛋白质,参与信号传导作用,此抗体识别大多数B细胞上的一种抗原。多用于标记B淋巴细胞及B细胞淋巴瘤。
CD20一般不与T细胞交叉反应,对B细胞淋巴瘤和急慢性淋巴细胞白血病等有较好的细胞特异性,因此在恶性淋巴瘤,尤其是T或B细胞淋巴瘤的分类上,CD20是最常用的B细胞标志。
产品图片
Sample:
Lane 1: Large intestine (Mouse) Lysate at 40 ug
Lane 2: Bone (Mouse) Lysate at 40 ug
Primary: Anti-CD20 (bs-20638R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-CD20  (bs-20638R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-CD20 (bs-20638R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Sample:
Spleen (Mouse)Lysate at 40 ug
Lymph node(Mouse)Lysate at 40 ug
Primary: Anti-CD20 (bs-20638R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 33kD
Observed band size: 35kD
Blank control(mouse): Mouse Spleen(fixed with 2% paraformaldehyde for 10 min at 37℃).
Primary Antibody:Rabbit Anti-CD20 antibody (bs-20638R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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