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说明书: 50ul  100ul  200ul
50ul/1138.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-2938R
英文名称 TdT
中文名称 末端脱氧核苷酸转移酶抗体
别    名 Deoxynucleotidyltransferase terminal; DNA nucleotidylexotransferase; DNTT; Nucleosidetriphosphate DNA deoxynucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal deoxyribonucleotidyltransferase; Terminal transferase; TDT_HUMAN.  
研究领域 细胞生物  免疫学  染色质和核信号  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Rat,  (predicted: Mouse, Dog, Horse, Rabbit, )
产品应用 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 58kDa
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human TdT/DNTT:351-450/509 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 TdT is a nuclear enzyme involved in the recombination of Immunoglobulin and T cell receptor genes. It is expressed in early lymphoid cells and by tumour cells in certain classes of acute lymphoid leukaemia.

Subunit:
Interacts with PRP19 and DNTTIP1. Forms a ternary complex with DNTTIP2 and core histone. Released from this complex by PCNA.

Subcellular Location:
Nucleus.

Similarity:
Belongs to the DNA polymerase type-X family.
Contains 1 BRCT domain.

SWISS:
P04053

Gene ID:
1791

Database links:

Entrez Gene: 1791 Human

Entrez Gene: 21673 Mouse

Entrez Gene: 294051 Rat

Omim: 187410 Human

SwissProt: P04053 Human

SwissProt: P09838 Mouse

Unigene: 534206 Human

Unigene: 25620 Mouse

Unigene: 143741 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TdT) Polyclonal Antibody, Unconjugated (bs-2938R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Blank control (Black line):Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-TdT antibody (bs-2938R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Molt-4 cells were fixed with 4% PFA for 10min at room temperature ,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TdT Antibody(bs-2938R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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