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说明书: 50ul  100ul  200ul
50ul/1098.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-6028R
英文名称 CD16
中文名称 FC段γ受体3/免疫球蛋白G Fc段受体III抗体
别    名 FCG3A_HUMAN; Low affinity immunoglobulin gamma Fc region receptor III-A; CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; CD_antigen: D16a; FCGR3A; CD16A; FCG3; FCGR3; IGFR3.  
Specific References  (3)     |     bs-6028R has been referenced in 3 publications.
[IF=] Yihan Lu  et al. Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy. Int Immunopharmacol. 2020 Aug;85:106563.  IF ;  Mouse.  
[IF=] Jing Liet al. hUC-MSCs ameliorated CUMS-induced depression by modulating complement C3 signaling-mediated microglial polarization during astrocyte-microglia crosstalk. Brain Res Bull . 2020 Oct;163:109-119.  IHC ;  mouse.  
[IF=2.38] Ding, Peng, et al. "Expression of Tumor-Associated Macrophage in Progression of Human Glioma." Cell Biochemistry and Biophysics (2014): 1-7.  IHC-P ;  Human.  
研究领域 细胞生物  免疫学  干细胞  细胞表面分子  细胞类型标志物  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Pig, Cow, Rabbit, Sheep, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 27kDa
细胞定位 细胞膜 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human IGFR3/CD16:131-230/254 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer(NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

Function:
Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.

Subunit:
Exists as a heterooligomeric receptor complex with Fc epsilon receptor I gamma subunit and / or the CD3 zeta subunit. Interacts with INPP5D/SHIP1.

Subcellular Location:
Cell membrane. Secreted. Exists also as a soluble receptor.

Tissue Specificity:
Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.

Post-translational modifications:
Glycosylated. Contains high mannose- and complex-type oligosaccharides.
The soluble form is produced by a proteolytic cleavage. [MISCELLANEOUS] Encoded by one of two nearly indentical genes: FCGR3A (Shown here) and FCGR3B which are expressed in a tissue-specific manner. The Phe-203 in III-A determines the transmembrane domains whereas the 'Ser-203' in III-B determines the GPI-anchoring.

Similarity:
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

SWISS:
P08637

Gene ID:
2214

Database links:

Entrez Gene: 2214 Human

Entrez Gene: 14131 Mouse

Entrez Gene: 304966 Rat

Omim: 146740 Human

SwissProt: P08637 Human

SwissProt: P08508 Mouse

SwissProt: P27645 Rat

Unigene: 372679 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample:
Lane 1: Thymus (Rat) Lysate at 40 ug
Lane 2: Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-CD16 (bs-6028R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 60 kD
Sample:
Raw264.7(Mouse) Cell Lysate at 30 ug
Primary: Anti- CD16 (bs-6028R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD16) Polyclonal Antibody, Unconjugated (bs-6028R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Cell: U937(4% Paraformaldehyde fixed for 10 minutes,2% BSA at 4∑blocked for 30 minutes.).
Concentration:1:100;Incubation: 40 minutes.
Flow cytometric analysis of Rabbit Anti-CD16 antibody (bs-6028R)(green) compared with control in the absence of primary antibody (blue) followed by U937.
Secondary antibody: Goat Anti-rabbit IgG/PE antibody (bs-0295G-PE)
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