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Rabbit Anti-Beta tubulin (Loading Control) antibody (bs-4511R)
~~~促销,代码KT1102~~~
~~~促销,代码KT1101~~~

~~~20周年感恩回馈“万能卡”~~~
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说明书: 50ul  100ul  500ul
50ul/680.00元
100ul/980.00元
500ul/4000.00元
大包装/询价

产品编号 bs-4511R
英文名称 Beta tubulin (Loading Control)
中文名称 微管蛋白β tubulin/Tubulin β(内参)抗体
别    名 Beta 4 tubulin; Tubulin-beta; Tubulin beta; Beta 5 tubulin; BetaTubulin; Beta-Tubulin; dJ40E16.7; TUBB; TUBB2; TUBB2A; TUBB5; tubulin beta 2A; Tubulin beta chain; Tubulin beta-5 chain; TUBB4A; TUBB4; Tubulin 5 beta; Tubulin beta-4 chain; TBB4A_HUMAN; Tubulin beta-4A chain.  
Specific References  (12)     |     bs-4511R has been referenced in 12 publications.
[IF=0] 朱镕鑫, et al. "Ghrelin 信号通路在有氧运动对高胆固醇血症小鼠心肌损伤保护中的作用."  WB ;  Mouse.  
[IF=3.266] Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019.  WB ;  Mouse.  
[IF=] Li-Hua Muet al. Antiangiogenic effects of AG36, a triterpenoid saponin from Ardisia gigantifolia stapf. J Nat Med . 2020 Sep;74(4):732-740.  WB ;  Human.  
[IF=6.583] Wu MM et al. Lovastatin attenuates renal tubule‐specific ABCA1 knockout‐induced hypertension by inhibiting the epithelial sodium channel. Br J Pharmacol. 2019 Jun 21.   WB ;  Mouse.  
[IF=4.88] Fang Q et al. Evaluation of a hybrid small caliber vascular graft in rabbit model.J Thorac Cardiovasc Surg. 2019 Mar 6. pii: S0022-5223(19)30550-1.  WB ;  Rabbit.  
[IF=4.106] Nikonorova IA et al. Age modulates liver responses to asparaginase-induced amino acid stress in mice. J Biol Chem. 2019 Aug 14. pii: jbc.RA119.009864.   WB ;  Mouse.  
[IF=3.115] Sun F et al. PKA/β2-AR-Gs/Gi signaling pathway is associated with anti-inflammatory and pro-apoptotic effects of Fuzi and Banxia combination on rats subjected to pressure overload. J Ethnopharmacol. 2019 May 10;235:375-384.  WB ;  Rat.  
[IF=2.7] Peng, Wang, et al. "Attenuation of specific CTL responses by highly efficient transduction of the recombinant adenovirus expressing His-tag-ICP47 fusion gene." Life Science Journal 10.1 (2013).  WB ;  Human.  
[IF=2.656] Liu et al. Effects of insulin-like growth factor binding protein 3 on apoptosis of cutaneous squamous cell carcinoma cells. (2018) Onco.Targets.Ther. 11:6569-6577  WB ;  
[IF=2.11] Yan, Lijie, et al. "Regulator of calcineurin 1-1L protects cardiomyocytes against hypoxia-induced apoptosis via mitophagy." Journal of cardiovascular pharmacology 64.4 (2014): 310-317.  WB ;  Human.  
[IF=2.1] Li, Xinxin, et al. "Long-term thermal manipulation in the late incubation period can inhibit breast muscle development by activating endoplasmic reticulum stress in duck (Anasplatyrhynchos domestica)." Journal of thermal biology 70.Pt B (2017): 37.  WB ;  Other Species.  
[IF=1.72] Li, Xinxin, et al. "Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signaling." Bioscience Reports (2014).  WB ;  
产品类型 体育娱乐网址平台 
研究领域 免疫学  神经生物学  细胞骨架  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Rabbit, )
产品应用 WB=1:5000-20000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test ICC=1:100 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 55kDa
细胞定位 细胞浆 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human tubulin Beta:61-160/444 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) and therefore beta Tubulin should not be used as loading control for these tissues.

Function:
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Subunit:
Dimer of alpha and beta chains. May interact with RNABP10 (By similarity). Interacts with PIFO. Interacts with MX1 (By similarity).

Subcellular Location:
Cytoplasm, cytoskeleton.

Tissue Specificity:
Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Post-translational modifications:
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable).

Similarity:
Belongs to the tubulin family.

SWISS:
P07437

Gene ID:
203068

Database links:

Entrez Gene: 203068 Human

Omim: 191130 Human

SwissProt: P07437 Human

SwissProt: P99024 Mouse

SwissProt: P69897 Rat




Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

结构蛋白(Structural Proteins)
tubulin是一种大量存在于哺乳动物脑组织中的微管亚基蛋白,在结构上是由两个极为相近的α和β亚基组成的二聚体、多聚体形成微管细丝,是微管的主要成分。
微管蛋白是球形分子, 有两种类型:α微管蛋白(α-tubulin)货号:bs-0195R和β微管蛋白(β-tubulin), 这两种微管蛋白具有相似的三维结构, 能够紧密地结合成二聚体, 作为微管组装的亚基。 α亚基由450个氨基酸组成, β亚基是由455个氨基酸组成, 这两种亚基有35~40%的氨基酸序列同源, 表明编码它们的基因可能是由同一原始祖先演变而来.
产品图片
Sample:
Cerebrum (Rat) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- Beta tubulin (bs-4511R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample:
Brain (mouse) Lysate at 40 ug
Heart (mouse) Lysate at 40 ug
Primary: Anti- beta tubulin(bs-4511R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary:
Anti-Beta tubulin (bs-4511R) at 1/1000~20000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary:
Anti-Beta tubulin (bs-4511R) at 1/1000~20000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample:
MDA-MB-231 (Human) Lysate at 40 ug
Primary:
Anti-Beta tubulin (bs-4511R) at 1/2000~20000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Cerebellum (Mouse) Lysate at 40 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
Cerebrum (Mouse) Lysate at 40 ug
HL60(Human) Cell Lysate at 30 ug
Cerebrum (Rat) Lysate at 40 ug
Cerebellum (Rat) Lysate at 40 ug
Heart (Mouse) Lysate at 40 ug
Primary: Anti- Beta tubulin (bs-4511R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample: Spleen (Mouse) Lysate at 40 ug
Primary: Anti- Beta tubulin (bs-4511R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample:
Heart (Mouse) Lysate at 40 ug
MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti-Beta tubulin (bs-4511R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Sample:
Brain (Rat) Lysate at 40 ug
Heart (Rat) Lysate at 40 ug
Primary: Anti-Beta tubulin (bs-4511R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 55 kD
Observed band size: 50 kD
Sample:
Lane 1: Cerebellum (Mouse) Lysate at 40 ug
Lane 2: Cerebellum (Rat) Lysate at 40 ug
Lane 3: Cerebrum (Mouse) Lysate at 40 ug
Lane 4: HL-60 (Human) Cell Lysate at 30 ug
Lane 5: Cerebrum (Rat) Lysate at 40 ug
Lane 6: Heart (Mouse) Lysate at 40 ug
Lane 7: Heart (Rat) Lysate at 40 ug
Primary: Anti-Beta tubulin (bs-4511R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Sample:
Lane 1: SiHa (Human) Cell Lysate at 30 ug
Lane 2: Cerebrum (Rat) Lysate at 40 ug
Lane 3: Cerebrum (Mouse) Lysate at 40 ug
Lane 4: Heart (Rat) Lysate at 40 ug
Lane 5: Heart (Mouse) Lysate at 40 ug
Primary: Anti- Beta tubulin (bs-4511R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 49 kD
Sample:
MCF-7(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
MDA-MB-231(Human) Cell Lysate at 30 ug
Primary: Anti-Beta tubulin (bs-4511R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 48 kD
Sample:
Uterus (Mouse) Lysate at 40 ug
Primary: Anti- Beta tubulin (bs-4511R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 53 kD
Sample:
Ovary (Mouse) Lysate at 40 ug
Primary: Anti- Beta tubulin (bs-4511R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 53 kD
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin(Loading Control)) Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta tubulin ) Polyclonal Antibody, Unconjugated (bs-4511R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell:Sh-sy5y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Beta tubulin) polyclonal Antibody, Unconjugated (bs-4511R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell:Sh-sy5y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Beta tubulin) polyclonal Antibody, Unconjugated (bs-4511R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control:HL-60.
Primary Antibody (green line): Rabbit Anti-Beta tubulin antibody (bs-4511R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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