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说明书: 50ul  100ul  200ul
50ul/1138.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-3792R
英文名称 CD21
中文名称 2型补体受体抗体
别    名 C3DR; CD 21; CD21 antigen; Complement C3d receptor; Complement component (3d/Epstein Barr virus) receptor 2; Complement component receptor 2; Complement receptor type 2; Cr 2; Cr2; EBV receptor; Epstein Barr virus receptor.  
研究领域 细胞生物  免疫学  细胞膜受体  细菌及病毒  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Rat,  (predicted: Mouse, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 Flow-Cyt=1μg/Test (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 111kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD21:981-1092/1092 <Cytoplasmic>
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 CD21 also known as complement receptor 2 (CR2), C3d receptor or EBV receptor is a 140 kDa protein. CD21 is a glycosylated type I transmembrane protein consisting of an extracellular face of a series of 15 or 16 CCP domains. CD21 is the receptor for complement components C3d and iC3b as well as the Epstein-Barr virus (EBV) glycoprotein gp350/220. The soluble CD21 (sCD21) was shown to efficiently trigger CD23 signalling pathways in human monocytes. By inducing release of proinflammatory cytokines and upregulating expression of molecules involved in antigen presentation, sCD21 modulates critical monocyte functions that may be relevant to allergic and inflammatory disorders.

Function:
Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation.

Subunit:
Interacts (via Sushi domain 1 and 2) with C3dg.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.

DISEASE:
Genetic variations in CR2 are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9) [MIM:610927]. Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Defects in CR2 are the cause of immunodeficiency, common variable, type 7 (CVID7) [MIM:614699]. A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.

Similarity:
Belongs to the receptors of complement activation (RCA) family.
Contains 15 Sushi (CCP/SCR) domains.

SWISS:
P20023

Gene ID:
1380

Database links:

Entrez Gene: 1380 Human

Entrez Gene: 12902 Mouse

Omim: 120650 Human

SwissProt: P20023 Human

SwissProt: P19070 Mouse

Unigene: 445757 Human

Unigene: 235387 Mouse





产品图片
Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-CD21 (bs-3792R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 111 kD
Observed band size: 111 kD
Blank control(blue): U-87MG Cells(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody: Rabbit Anti-CD21/AF647 Conjugated antibody (bs-3792R/AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody: Rabbit Anti-CD21/AF647 Conjugated antibody (bs-3792R/AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Blank control: Raji cells(blue).
Primary Antibody:Rabbit Anti- CD21 antibody(bs-3792R), Dilution: 1μg in 100 1μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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