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说明书: 50ul  100ul  200ul
50ul/1180.00元
100ul/1980.00元
200ul/3200.00元
大包装/询价

产品编号 bs-2590R
英文名称 NRSF
中文名称 神经元抑制蛋白抗体
别    名 RE1-Silencing Transcription Factor; NRSF; Neural-Restrictive Silencer Factor; XBR; Neuron Restrictive Silencer Factor; Repressor Binding To The X2 Box; X2 Box Repressor; REST_HUMAN  
Specific References  (2)     |     bs-2590R has been referenced in 2 publications.
[IF=2.472] Zhu NW et al. Possible mechanisms of lycopene amelioration of learning and memory impairment in rats with vascular dementia. Neural Regen Res. 2020 Feb;15(2):332-341.   WB&IHC-P ;  Rat.  
[IF=] Buqing Yeet al. The chromatin remodeler SRCAP promotes self‐renewal of intestinal stem cells. EMBO J . 2020 Jul 1;39(13):e103786.  IHC, IP, IF ;  mouse.  
研究领域 神经生物学  锌指蛋白  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse,  (predicted: Rat, Chicken, Pig, Cow, Horse, Rabbit, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 121kDa
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human NRSF:401-450/1097 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 This gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described [provided by RefSeq, Jul 2010]

Function:
Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression.

Subunit:
Interacts with SIN3A, SIN3B and RCOR1. Interacts with CDYL. Interacts with EHMT1 and EHMT2 only in the presence of CDYL. Part of a complex containing at least CDYL, REST, WIZ, SETB1, EHMT1 and EHMT2.

Subcellular Location:
Nucleus.

Tissue Specificity:
Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary.

Similarity:
Contains 9 C2H2-type zinc fingers.

SWISS:
Q13127

Gene ID:
5978

Database links:

Entrez Gene: 5978 Human

Entrez Gene: 19712 Mouse

Entrez Gene: 83618 Rat

GenBank: AAB17211 Human

Omim: 600571 Human

SwissProt: Q13127 Human

SwissProt: Q155C6 Mouse

SwissProt: Q8VIG1 Mouse

SwissProt: O54963 Rat

SwissProt: O54964 Rat

Unigene: 307836 Human

Unigene: 631513 Human

Unigene: 28840 Mouse

Unigene: 482359 Mouse

Unigene: 10879 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample:
Hela(Human) Cell Lysate at 30 ug
Hela KO NRSF (Human) Cell Lysate at 30 ug
Primary: Anti- NRSF (bs-2590R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kD
Observed band size: 121 kD
Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-NRSF (bs-2590R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 121 kD
Observed band size: 121 kD
NRSF was immunoprecipitated from human hela cells lysate with bs-2590R at 1/150 dilution. Western blot was performed from the immunoprecipitate using protein A/G beads. HRP Conjugated Mouse anti-Rabbit IgG (Light Chain specific) was used as secondary antibody at 1:5000 dilution.
Lane 1: human hela cells lysate 10 µg (Input).
Lane 2: bs-2590R IP in human hela cells lysate.
Lane 3: native rabbit IgG IP in human hela cells lysate (negative control).
Secondary
All lanes : Mouse anti-Rabbit IgG (Light Chain specific), HRP Conjugated, 1:5000
Tissue/cell: human gastric tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NRSF/REST Polyclonal Antibody, Unconjugated(bs-2590R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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