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说明书: 50ul  100ul  200ul
50ul/1098.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-0458R
英文名称 Thioredoxin
中文名称 硫氧还蛋白抗体
别    名 ADF; Surface associated sulphydryl protein; Thioredoxin-1; Thioredoxin 1; Thioredoxin1; TXN protein; ADF; ATL derived factor; DKFZp686B1993; MGC61975; SASP; Surface associated sulphydryl protein; Surface-associated sulphydryl protein; Thioredoxin; TRDX; TRX 1; TRX; TRX1; TXN; TXN; ATL derived factor; ATL-derived factor; THIO_HUMAN; zgc:92903.  
Specific References  (3)     |     bs-0458R has been referenced in 3 publications.
[IF=7.6] Gao, Fuping, et al. "Cytotoxicity and therapeutic effect of irinotecan combined with selenium nanoparticles." Biomaterials (2014).  IHC-P ;  Mouse.  
[IF=3.24] Li, Mo-fei, et al. "First characterization of a teleost Epstein-Barr virus-induced gene 3 (EBI3) reveals a regulatory effect of EBI3 on the innate immune response of peripheral blood leukocytes." Developmental & Comparative Immunology 41.4 (2013): 514-522.  WB ;  
[IF=1.55] Zhang, Junjie, et al. "Screening and identification of post-traumatic stress-related serum factors in children with Wilms tumors." Oncology Letters 11.2 (2016): 1299-1304.  WB ;  Human.  
研究领域 肿瘤  细胞生物  免疫学  信号转导  转录调节因子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Rabbit, fusion protein)
产品应用 WB=1:1000-5000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 12kDa
细胞定位 细胞核 细胞浆 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Thioredoxin:51-105/105 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Function:
Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Subunit:
Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. In case of infection, interacts with S.typhimurium protein slrP. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA-binding activity in a redox-dependent manner.

Subcellular Location:
Nucleus. Cytoplasm. Secreted. Note=Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.

Similarity:
Belongs to the thioredoxin family. Contains 1 thioredoxin domain.

SWISS:
P10599

Gene ID:
7295

Database links:

Entrez Gene: 7295 Human

Entrez Gene: 22166 Mouse

Entrez Gene: 116484 Rat

Omim: 187700 Human

SwissProt: P10599 Human

SwissProt: P10639 Mouse

SwissProt: P11232 Rat

Unigene: 435136 Human

Unigene: 260618 Mouse

Unigene: 29777 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

硫氧还蛋白(Thioredoxin,Trx)是一类广泛存在于生物体内的多功能酸性蛋白,分子量约12kDa,是一种小分子含硒蛋白质,参与细胞的一系列生化反应,包括酶活性的调节、转录因子的调控等,是重要的酶活力调节蛋白。
硫氧还蛋白的功能绝大多数是依赖于硫氧还蛋白还原靶蛋白中的二硫键。所有硫氧还蛋白均有一个保守的活性中心,Trp-Cys-Gly(Ala)-Pro-Cys,活性中心有两个具有氧还活性的半胱氨酸残基。
产品图片
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thioredoxin) Polyclonal Antibody, Unconjugated (bs-0458R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thioredoxin) Polyclonal Antibody, Unconjugated (bs-0458R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Thioredoxin) Polyclonal Antibody, Unconjugated (bs-0458R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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