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产品编号 bs-0648R
英文名称 CD8
中文名称 CD8抗体
别    名 CD8 antigen, alpha polypeptide (p32); CD8a; CD8a antigen; CD8a molecule; CD8A_MOUSE; Leu2; Leu2 T lymphocyte antigen; MAL; OKT8 T cell antigen; p32; T cell antigen Leu2; T cell co receptor; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T cell antigen.  
Specific References  (41)     |     bs-0648R has been referenced in 41 publications.
[IF=1.26] Jaime-Ramirez, Alena C., et al. "Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma." Molecular Therapy-Oncolytics 5 (2017): 87-96.  IHC-P ;  Mouse.  
[IF=1.26] Jaime-Ramirez et al. Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma. (2017) Mol.Ther.Oncolytics. 5:87-96  IHC-P ;  Mouse.  
[IF=5.345] Yuan SJ et al. Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer.J Nanobiotechnology. 2019 Oct 17;17(1):110.   IHC-P ;  Mouse.  
[IF=] Yihan Lu  et al. Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy. Int Immunopharmacol. 2020 Aug;85:106563.  IF ;  Mouse.  
[IF=] Zhao B et al. A TCM Formula YYWY Inhibits Tumor Growth in Non-Small Cell Lung Cancer and Enhances Immune-Response Through Facilitating the Maturation of Dendritic Cells. Front Pharmacol. 2020 Jun 9;11:798.  IHF-P ;  Mouse.  
[IF=8.39] Wang, Raymond M., et al. "Humanized mouse model for assessing the human immune response to xenogeneic and allogeneic decellularized biomaterials." Biomaterials (2017).  IHC ;  Mouse.  
[IF=7.786] Zhu,et al.Co-delivery of tumor antigen and dual toll-like receptor ligands into dendritic cell by silicon microparticle enables efficient immunotherapy against melanoma.(2018) Journal of Controlled Release. 272:72-82.  IHC-P ;  Mouse.  
[IF=] Senbiao Chenaet al. Carrier-free nanoassembly of doxorubicin prodrug and siRNA for combinationally inducing immunogenic cell death and reversing immunosuppression. Nano Today 35 (2020) 100924  IHC ;  mouse.  
[IF=] Boon Ching Teeet al. Xenogeneic mesenchymal stem cell transplantation for mandibular defect regeneration. Xenotransplantation . 2020 Sep;27(5):e12625.  IHC ;  rat.  
[IF=6.493] Ashraf et al. Exogenous Lipocalin 2 Ameliorates Acute Rejection in a Mouse Model of Renal Transplantation. (2016) Am.J.Transplant. 16:808-20  IHC-P ;  Mouse.  
[IF=6.28] Kroner, Jochen, et al. "Mast cells are critical regulators of bone fracture‐induced inflammation and osteoclast formation and activity." Journal of Bone and Mineral Research (2017).  IHC-P ;  Mouse.  
[IF=5.68] Ashraf, M. I., et al. "Exogenous Lipocalin 2 Ameliorates Acute Rejection in a Mouse Model of Renal Transplantation." American Journal of Transplantation (2015).  IHC-P ;  Mouse.  
[IF=5.595] Rapp et al. Induced global deletion of glucocorticoid receptor impairs fracture healing. (2018) FASEB.J. 32:2235-2245  IHC ;  Mouse.  
[IF=5.5] Simanovich et al. Inhibition of tumor growth and metastasis by EMMPRIN multiple antigenic peptide (MAP) vaccination is mediated by immune modulation. (2017) Oncoimmunology. 6:e1261778  IHC ;  Mouse.  
[IF=4.982] Demontoux L et al. Hypotonic stress enhances colon cancer cell death induced by platinum derivatives and immunologically improves anti‐tumor efficacy of intraperitoneal chemotherapy. Int J Cancer. 2019 Jul 25.  IHC-P ;  Mouse.  
[IF=4.929] Wang et al. Targeted Disruption of the β2-Microglobulin Gene Minimizes the Immunogenicity of Human Embryonic Stem Cells. (2015) Stem.Cells.Transl.Me. 4:1234-45  IF(ICC) ;  Human.  
[IF=4.56] Kovtun, A., et al. "The crucial role of neutrophil granulocytes in bone fracture healing." European Cells and Materials 32 (2016):152-162.  IHC-P ;  Mouse.  
[IF=4.539] Kanai K et al. Murine γ-Herpesvirus 68 Induces Severe Lung Inflammation in IL-27-Deficient Mice with Liver Dysfunction Preventable by Oral Neomycin. J Immunol. 2018 Apr 15;200(8):2703-2713.  IHC-P ;  Mouse.  
[IF=4.213] Wan WJ et al. Doxorubicin and siRNA-PD-L1 co-delivery with T7 modified ROS-sensitive nanoparticles for tumor chemoimmunotherapy. Int J Pharm. 2019 Jul 20;566:731-744.   IHF ;  Mouse.  
[IF=4.2] Kovtun, Anna, et al. "Complement receptors C5aR1 and C5aR2 act differentially during the early immune response after bone fracture but are similarly involved in bone repair." Scientific Reports 7.1 (2017): 14061.  IHC-P ;  Mouse.  
[IF=4.069] Mödinger Y et al. Reduced Terminal Complement Complex Formation in Mice Manifests in Low Bone Mass and Impaired Fracture Healing. (2018) Am J Pathol. Oct 17.   IHC ;  Mouse.  
[IF=4] Prystaz, Katja, et al. "Distinct effects of interleukin-6 classic and trans-signaling in bone fracture healing." The American Journal of Pathology.  IHC-P ;  Mouse.  
[IF=4] Wang et al. Targeted Disruption of the β2-Microglobulin Gene Minimizes the Immunogenicity of Human Embryonic Stem Cells. (2015) Stem.Cells.Transl.Me. 4:1234-45  IF(ICC) ;  Human.  
[IF=3.73] Lee M-F, Yang K-J, Wang NM, Chiu Y-T, Chen P-C, et al. (2014) The Development of a Murine Model for Forcipomyia taiwana (Biting Midge) Allergy. PLoS ONE 9(3): e91871  IHC-P ;  Mouse.  
[IF=3.69] Li, Yan, et al. "Immunogenic FEAT protein circulates in the bloodstream of cancer patients." Journal of Translational Medicine 14.1 (2016): 275.  IHC-P ;  Mouse.  
[IF=3.64] He, Qiushan, et al. "Low-dose paclitaxel enhances the anti-tumor efficacy of GM-CSF surface-modified whole-tumor-cell vaccine in mouse model of prostate cancer." Cancer Immunology, Immunotherapy 60.5 (2011): 715-730.  IHC-P ;  Mouse.  
[IF=3.285] Zhang H et al. Utilizing VEGF165b mutant as an effective immunization adjunct to augment antitumor immune response.Vaccine. 2019 Apr 3;37(15):2090-2098.  IHC-P ;  Mouse.  
[IF=3.26] Tanaka, Rui, et al. "The efficacy of combination therapy with oncolytic herpes simplex virus HF10 and dacarbazine in a mouse melanoma model." American Journal of Cancer Research 7.8 (2017): 1693.  IHC-P ;  Mouse.  
[IF=3.06] Zhang, Wen, et al. "The role of CXCR3 in the induction of primary biliary cirrhosis." Clinical and Developmental Immunology 2011 (2011).  IHC-P ;  Mouse.  
[IF=3.03] Willett, Emily S., et al. "Standardized Rat Model Testing Effects of Inflammation and Grafting on Extraction Healing." Journal of Periodontology (2017): 1-15.  IHC ;  Rat.  
[IF=2.885] Schmid et al. Acute Renal Graft-Versus-Host Disease in a Murine Model of Allogeneic Bone Marrow Transplantation. (2017) Cell.Transplant. 26:1428-1440  IHC ;  Mouse.  
[IF=2.829] Wang,et al.Black tea and D. candidum extracts play estrogenic activity via estrogen receptor α-dependent signaling pathway.(2018) American Journal of Translational Research. 10:114-125.  WB ;  Human.  
[IF=2.49] Ju et al. An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model. (2016) BMC.Immunol. 17:31  IHC ;  Mouse.  
[IF=2.37] Qiao, Xinwei, et al. "Increased expression of Tim-3 and its ligand Galectin-9 in rat allografts during acute rejection episodes." Biochemical and biophysical research communications 445.3 (2014): 542-548.  IHC-F ;  Rat.  
[IF=2.232] Dong et al. Antitumor effect of embryonic stem cells in a non-small cell lung cancer model: antitumor factors and immune responses. (2013) Int.J.Med.Sc. 10:1314-20  IHC-P ;  Mouse.  
[IF=2.16] Ju, Huanyu, et al. "An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model." BMC immunology 17.1 (2016): 31.  IHC-P ;  Mouse.  
[IF=1.99] Chuang, Meng-Hsien, et al. "The Chinese medicine JC-001 enhances the chemosensitivity of Lewis lung tumors to cisplatin by modulating the immune response." BMC Complementary and Alternative Medicine 17.1 (2017): 210.  IHC-P ;  Mouse.  
[IF=1.69] Jeong, Keun-Yeong, et al. "Combination of macrophage inflammatory protein 1 alpha with existing therapies to enhance the antitumor effects on murine hepatoma." Journal of Radiation Research (2014): rru077.  WB ;  Mouse.  
[IF=1.655] Takamori S et al. HMGB1 blockade significantly improves luminal fibrous obliteration in a murine model of bronchiolitis obliterans syndrome.Takamori S et al.  IHC-P ;  Mouse.  
[IF=1.448] Sun J1 et al. Clinical effects of lentinan combined with budesonide inhalation in treating acute exacerbation of chronic obstructive pulmonary disease under mechanical ventilation. Exp Ther Med. 2019 Mar;17(3):1503-1508.   FCM ;  Human.  
[IF=1.41] Haffner-Luntzer, Melanie, et al. "The inflammatory phase of fracture healing is influenced by oestrogen status in mice." European Journal of Medical Research 22.1 (2017): 23.  FCM ;  Mouse.  
研究领域 细胞生物  免疫学  干细胞  细胞表面分子  细胞类型标志物  淋巴细胞  t-淋巴细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, 
产品应用 WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 27kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse CD8:51-150/247 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011].

Function:
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Subunit:
In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Similarity:
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:
P01731

Gene ID:
12525

Database links:

Entrez Gene: 925 Human

Entrez Gene: 926 Human

Entrez Gene: 12525 Mouse

Entrez Gene: 12526 Mouse

Omim: 186910 Human

SwissProt: P01732 Human

SwissProt: P10966 Human

SwissProt: P01731 Mouse

SwissProt: P10300 Mouse

Unigene: 405667 Human

Unigene: 85258 Human

Unigene: 1858 Mouse

Unigene: 333148 Mouse




Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti- CD8 (bs-0648R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 36 kD
Sample:Lymph node(Mouse) Lysate at 30 ug
Primary: Anti-CD8(bs-0648R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 32 kD
Image kindly submitted by Amy Beck as part of the free sample program. Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-CD8 Polyclonal Antibody, Unconjugated (bs-0648R) at 1:250.
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