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产品编号 bs-1309R
英文名称 Syndecan-1
中文名称 多配体蛋白聚糖1抗体
别    名 SDC1; SDC; SYND1; Syndecan I; SyndecanI; syndecan; Sdc1; Syndecan1; AA408134; AA409076; Sstn; syn-1; CD138; CD 138; CD-138; CD138 antigen; Heparan sulfate proteoglycan fibroblast growth factor receptor; SDC 1; Syndecan1 precursor; SDC1_HUMAN.  
Specific References  (5)     |     bs-1309R has been referenced in 5 publications.
[IF=] Linlin Yanget al. CAMKIIγ is a targetable driver of multiple myeloma through CaMKIIγ/ Stat3 axis. Aging (Albany NY) . 2020 Jul 13;12(13):13668-13683.  IHC, IF ;  Human.  
[IF=2.97] Hambruch, N., et al. "Bovine placentomal heparanase and syndecan expression is related to placental maturation." Placenta.  IHC-P ;  Bovine.  
[IF=2.65] Miyatani, Kozo, et al. "A high number of IgG4-positive cells in gastric cancer tissue is associated with tumor progression and poor prognosis." Virchows Archiv (2016): 1-9.  IHC-F ;  Human.  
[IF=2.54] Chen, Song, et al. "Heparanase Mediates Intestinal Inflammation and Injury in a Mouse Model of Sepsis." Journal of Histochemistry & Cytochemistry (2017): 0022155417692536.  IHC-P ;  Mouse.  
[IF=1.396] Xiong Y et al. Functions of T-cell subsets and their related cytokines in the pathological processes of autoimmune encephalomyelitic mice.(2018) Int J Clin Exp Pathol;11(10):4817-4826.  FC&IHC ;  Mouse.  
研究领域 肿瘤  神经生物学  细胞膜受体  细胞粘附分子  细胞表面分子  糖蛋白  上皮细胞  内皮细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Rat, Cow,  (predicted: Mouse, Chicken, Dog, Pig, Horse, Rabbit, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 34kDa
细胞定位 细胞膜 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD138:201-310/310 <Cytoplasmic>
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008].

Function:
Cell surface proteoglycan that bears both heparin sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.

Subunit:
Interacts with CDCP1.

Subcellular Location:
Membrane; Single-pass type I membrane protein. Secreted. Note=Shedding of the ectodomain produces a soluble form.

Post-translational modifications:
Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.

Similarity:
Belongs to the syndecan proteoglycan family.

SWISS:
P18827

Gene ID:
6382

Database links:

Entrez Gene: 6382 Human

Entrez Gene: 20969 Mouse

Entrez Gene: 25216 Rat

Omim: 186355 Human

SwissProt: P18827 Human

SwissProt: P18828 Mouse

SwissProt: P26260 Rat

Unigene: 224607 Human

Unigene: 665958 Human

Unigene: 2580 Mouse

Unigene: 11176 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Syndecan1(CD138)属于Syndecans基因家族,该家族是一类细胞表面跨膜蛋白多糖 ,是膜表面粘附受体 ,参与细胞-细胞外基质之间的粘附。
CD138表达于上皮细胞、胚胎间质细胞、血管平滑肌细胞、内皮细胞和神经细胞基底膜。Syndecan-1与恶性肿瘤的发生发展、浸润及转移有关,着色于胞浆和胞膜.
产品图片
Sample:
JEG-3(Human) Cell Lysate at 30 ug
Primary: Anti-Syndecan-1 (bs-1309R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (bs-1309R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (bs-1309R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Syndecan-1) Polyclonal Antibody, Unconjugated (bs-1309R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).
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