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产品编号 bs-1014R
英文名称 CD11b
中文名称 整合素αM/巨噬细胞表面分子抗体
别    名 Antigen CD11b p170; CD11 antigen like family member B; CD11b antigen; CD49d; Cell surface glycoprotein MAC 1 alpha subunit; Cell Surface Glycoprotein MAC1 Alpha Subunit; Complement Component Receptor 3 Alpha; CR 3 alpha chain; CR3 Alpha Chain; CR3A; Integrin Alpha M; integrin alpha M complement component 3 receptor 3 subunit; Leukocyte Adhesion Receptor MO1; MAC 1; MAC1A; MGC117044; MO1A; Neutrophil Adherence Receptor; Cd11b; CD11b/CD18; F730045J24Rik; Ly-40; ITGAM; CR3A;complement receptor type 3; CR3;ITAM_HUMAN.  
Specific References  (16)     |     bs-1014R has been referenced in 16 publications.
[IF=] Ye Hea et al. Improved osteointegration by SEW2871-encapsulated multilayers on micro-structured titanium via macrophages recruitment and immunomodulation. Applied Materials Today 20 (2020) 100673  IHC ;  Rat.  
[IF=] Zhixiong Li et al. Shuxuening injection facilitates neurofunctional recovery via down-regulation of G-CSF-mediated granulocyte adhesion and diapedesis pathway in a subacute stroke Mousemodel. Biomed Pharmacother. 2020 Jul;127:110213.  IHC ;  Mouse.  
[IF=41.577] Yamaguchi T et al. "Interspecies organogenesis generates autologous functional islets." Nature. 2017 Feb 9;542(7640):191-196.  IHF ;  Mouse&Rat.  
[IF=] Feng Y et al. Long noncoding RNA HOXA-AS2 functions as an oncogene by binding to EZH2 and suppressing LATS2 in acute myeloid leukemia (AML)Cell Death Dis.2020 Dec 2;11(12):1025.  IHC ;  Mouse.  
[IF=5.712] Bronzuoli MR et al. Neuroglia in the autistic brain: evidence from a preclinical model. Mol Autism. 2018 Dec 27;9:66.   WB ;  Rat.  
[IF=5.58] Zhang, Qian-Qian, et al. "CD11b deficiency suppresses intestinal tumor growth by reducing myeloid cell recruitment." Scientific reports 5 (2015).  IF(IHC-P) ;  Mouse.  
[IF=4.648] Long et al. Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells. (2017) Aging.Di. 8:301-313  FCM ;  Rat.  
[IF=3.31] Yu, Keke, et al. "Ethanol Supports Macrophage Recruitment and Reinforces Invasion and Migration of Lewis Lung Carcinoma." Alcoholism: Clinical and Experimental Research 38.10 (2014): 2597-2606.  IHC-P ;  Mouse.  
[IF=3.3] Velázquez, Kandy T., et al. "MicroRNA-155 deletion promotes tumorigenesis in the azoxymethane-dextran sulfate sodium model of colon cancer."American Journal of Physiology-Gastrointestinal and Liver Physiology 310.6 (2016): G347-G358.  WB ;  Mouse.  
[IF=3.3] Velázquez, Kandy T., et al. "MicroRNA-155 deletion promotes tumorigenesis in the azoxymethane-dextran sulfate sodium model of colon cancer."American Journal of Physiology-Gastrointestinal and Liver Physiology 310.6 (2016): G347-G358.  Mouse.  
[IF=3.125] Tatebayashi K et al. Adipose-derived stem cell therapy inhibits the deterioration of cerebral infarction by altering macrophage kinetics. Brain Res. 2019 Feb 2. pii: S0006-8993(19)30061-7.  IHC-P&IF ;  Mouse.  
[IF=2.939] Wei S et al. Overexpression of Toll-like receptor 4 enhances LPS-induced inflammatory response and inhibits Salmonella Typhimurium growth in ovine macrophages.(2018) Eur J Cell Biol.   ICF ;  sheep.  
[IF=2.93] Liu, Tao, et al. "Gr-1+ CD11b+ cells facilitate Lewis lung cancer recurrence by enhancing neovasculature after local irradiation." Scientific Reports 4 (2014).  IHC-P ;  Mouse.  
[IF=2.88] Long, Qianfa, et al. "Genetically engineered bone marrow mesenchymal stem cells improve functional outcome in a rat model of epilepsy." Brain Research (2013).  Rat.  
[IF=2.53] Kobayashi, Aya, et al. "AG490, a Jak2 inhibitor, suppressed the progression of murine ovarian Cancer." European Journal of Pharmacology (2015).  IHC-P ;  Mouse.  
[IF=1.69] Yamaguchi et al. Suppression of hepatic dysfunction in tenascin‑X‑deficient mice fed a high‑fat diet. (2017) Mol.Med.Rep. 16:4061-4067  IF(IHC-F) ;  Mouse.  
研究领域 细胞生物  免疫学  神经生物学  信号转导  细胞凋亡  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse,  (predicted: Rat, Pig, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 125kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse CD11b:77-180/1152 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Function:
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.

Subcellular Location:
Membrane.

Tissue Specificity:
Predominantly expressed in monocytes and granulocytes.

Similarity:
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6). Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.

SWISS:
P05555

Gene ID:
16409

Database links:

Entrez Gene: 3684 Human

Entrez Gene: 16409 Mouse

Omim: 120980 Human

SwissProt: P11215 Human

SwissProt: P05555 Mouse

Unigene: 172631 Human

Unigene: 262106 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片
Sample: Serum (mouse) Lysate at 40 ug
Primary: Anti- CD11b (bs-1014R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 125 kD
Observed band size: 140 kD
Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD11b/Integrin αM/Integrin Alpha M Polyclonal Antibody, Unconjugated (bs-1014R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: U937 (blue).
Primary Antibody:Rabbit Anti- CD11b antibody(bs-1014R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (bs-1014R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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