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Rabbit Anti-ATPase Na+/ K+ beta 2(Loading Control) antibody (bs-1152R)
~~~促销,代码KT1101~~~

~~~20周年感恩回馈“万能卡”~~~
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说明书: 50ul  100ul  500ul
50ul/680.00元
100ul/980.00元
500ul/4000.00元
大包装/询价

产品编号 bs-1152R
英文名称 ATPase Na+/ K+ beta 2(Loading Control)
中文名称 钠钾ATP酶通道蛋白(内参)抗体
别    名 adhesion molecule on glia; Na+K+ATPase; AMOG; AT1B2; AT1B2_HUMAN; ATP1B2; ATPase Na+/K+ transporting beta 2 polypeptide; ATPB2; ATPB2S; MGC93648; Na+/K+ -ATPase beta 2 subunit; Na, K ATPase beta 2 polypeptide; RATATPB2S; sodium potassium ATPase subunit beta 2 (non-catalytic); sodium pump subunit beta 2; sodium/potassium dependent ATPase beta 2 subunit; Sodium/potassium dependent ATPase subunit beta 2; sodium/potassium transporting ATPase beta 2 chain; sodium/potassium transporting ATPase subunit beta 2; Sodium/potassium-dependent ATPase subunit beta-2; Sodium/potassium-transporting ATPase subunit beta-2.  
Specific References  (1)     |     bs-1152R has been referenced in 1 publications.
[IF=1.689] Wang HY et al. Occludin Endocytosis is Involved in Disruption of the Intestinal Epithelial Barrier in a Mouse Model of Alcoholic Steatohepatitis. J Dig Dis. 2019 Jul 12.   WB ;  Human.  
产品类型 体育娱乐网址平台 
研究领域 通道蛋白  糖蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse,  (predicted: Rat, Pig, Cow, Horse, Rabbit, Guinea Pig, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 33kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ATP1b2:201-290/290 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 2 subunit. [provided by RefSeq, Jul 2008]

Function:
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known.

Subunit:
Composed of three subunits: alpha (catalytic), beta and gamma.

Subcellular Location:
Membrane; Single-pass type II membrane protein.

Similarity:
Belongs to the X(+)/potassium ATPases subunit beta family.

SWISS:
P14415

Gene ID:
482

Database links:

Entrez Gene: 482 Human

Omim: 182331 Human

SwissProt: P14415 Human

Unigene: 643540 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

通道蛋白(Channel Protein)
钠钾ATP酶是位于细胞膜上的一种糖蛋白,与ATP 的分解和细胞内外钠、钾离子的转运密切相关,哺乳动物各种组织细胞的钠钾ATP 酶的免疫学特性基本相同。
产品图片
Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-ATPase Na+/ K+ beta 2 (bs-1152R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Sample: NIH/3T3(mouse) Cell Lysate at 40 ug
Primary: Anti-ATPaseNa+/K+ beta2 (bs-1152R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AMOG Polyclonal Antibody, Unconjugated(bs-1152R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ATP1b2/Na+K+ATPase Polyclonal Antibody, Unconjugated(bs-1152R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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