扫码关注公众号           扫码咨询技术支持
客服热线:400-901-9800  客服QQ:4009019800  技术答疑  技术支持  质量反馈  人才招聘  关于我们  联系我们
体育娱乐网址平台_首页
首页 > 产品中心 > 一抗 > 产品信息
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购传真:010-58129612
技术支持:techsupport@bioss.com.cn
说明书: 50ul  100ul  200ul
50ul/1098.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-0269R
英文名称 FoxP3
中文名称 叉头蛋白P3抗体
别    名 AIID; AIID; DIETER; DIETER; Forkhead box P3; Forkhead box protein P3; FOXP3_HUMAN; FOXP3delta7; Immune dysregulation polyendocrinopathy enteropathy X linked; Immunodeficiency polyendocrinopathy enteropathy X linked; IPEX; IPEX; JM2; JM2; MGC141961; MGC141963; OTTHUMP00000025832; OTTHUMP00000025833; OTTHUMP00000226737; PIDX; PIDX; SCURFIN; SCURFIN; XPID; XPID.  
Specific References  (1)     |     bs-0269R has been referenced in 1 publications.
[IF=3.208] Matsubara et al. Immune activation during the implantation phase causes preeclampsia-like symptoms via the CD40-CD40 ligand pathway in pregnant mice. (2016) Hypertens.Re. 39:407-14  IHC ;  Mouse.  
研究领域 转录调节因子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Rat,  (predicted: Mouse, )
产品应用 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt= 3μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 47kDa
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human FoxP3:11-100/431 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].

Function:
Probable transcription factor. Plays a critical role in the control of immune response.

Subunit:
Interacts with IKZF3.

Subcellular Location:
Nucleus (Potential).

Post-translational modifications:
Acetylation on lysine residues stabilizes FOXP3 and promotes differentiation of T-cells into induced regulatory T-cells (iTregs) associated with suppressive functions. Deacetylated by SIRT1.

DISEASE:
Defects in FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX) [MIM:304790]; also known as X-linked autoimmunity-immunodeficiency syndrome. IPEX is characterized by neonatal onset insulin-dependent diabetes mellitus, infections, secretory diarrhea, trombocytopenia, anemia and eczema. It is usually lethal in infancy.

Similarity:
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain.

SWISS:
Q9BZS1

Gene ID:
50943

Database links:

Entrez Gene: 50943 Human

Entrez Gene: 20371 Mouse

Entrez Gene: 317382 Rat

Omim: 300292 Human

SwissProt: Q9BZS1 Human

SwissProt: Q99JB6 Mouse

SwissProt: D3ZKI1 Rat

Unigene: 247700 Human

Unigene: 182291 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

叉头蛋白3(FOXP3)是FOX蛋白家族成员之一,主要为T细胞转录蛋白,主要表达于T细胞+CD4+CD25,并调节该类T细胞的发育和功能.
FOXP3的表达受转化生长因子-β雌激素和糖皮质激素等调节,通过竞争性抑制活化T细胞核因子的转录活性而发挥作用.自身免疫性糖尿病患者体内CD4+CD25+T细胞减少,诱导FOXP3的表达或过继转移CD4+CD25+T细胞有可能预防自身免疫性糖尿病
产品图片
Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FoxP3) Polyclonal Antibody, Unconjugated (bs-0269R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FoxP3) Polyclonal Antibody, Unconjugated (bs-0269R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Blank control (Black line): Mouse spleen (Black).
Primary Antibody (green line): Rabbit Anti-FoxP3 antibody (bs-0269R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA goat serum to block non-specific protein-protein interactions for 15 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
版权所有 2019-2022 体育娱乐网址平台
通过国际质量管理体系ISO9001:2008 GB/T19001-2008认证    编号:00115Q211807R1M/1100
京ICP备05066980号-1         京公网安备110107000727号