扫码关注公众号           扫码咨询技术支持
客服热线:400-901-9800  客服QQ:4009019800  技术答疑  技术支持  质量反馈  人才招聘  关于我们  联系我们
体育娱乐网址平台_首页
首页 > 产品中心 > 一抗 > 产品信息
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购传真:010-58129612
技术支持:techsupport@bioss.com.cn
说明书: 50ul  100ul  200ul
50ul/1098.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-0558R
英文名称 EMP-1
中文名称 表皮膜蛋白1抗体
别    名 CL-20; EMP 1; EMP1; EMP1_HUMAN; Epithelial membrane protein 1; Protein B4B; TMP; Tumor-associated membrane protein.  
研究领域 细胞生物  免疫学  细胞凋亡  细胞膜受体  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Rat,  (predicted: Mouse, )
产品应用 ELISA=1:5000-10000 IHC-F=1:100-500 Flow-Cyt=3ug/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 17kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse EMP-1:101-160/160 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Epithelial membrane protein-1 (EMP-1) is a four pass transmembrane protein consisting of 160 amino acids. It is a member of a small family of epithelial membrane proteins. EMP-1 is very similar in structure to its close relative, Peripheral Myelin Protein 22 (PMP22). It is most predominantly expressed in tissues of the gastrointestinal tract but has also been found to be a junctional protein in the liver expressed along the intercellular border. EMP-1 directly interacts with the C-terminus of the P2X7 receptor and may be involved in membrane blebbing. EMP-1 may also be an important regulator in cell communication, signaling, and adhesion. When EMP-1 is overexpressed, cell proliferation is inhibited, S phase is arrested and G1 phase is prolonged in esophogeal cancer cells. EMP-1 may play a role in tumorigenesis and has been identified as a biomarker for gefitinib treatment resistance for patients with lung cancer.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Most prominently found in the gastrointestinal tract, skin, lung, and brain but not in liver.

Similarity:
Belongs to the PMP-22/EMP/MP20 family.

SWISS:
P47801

Gene ID:
13730

Database links:

Entrez Gene: 2012 Human

Omim: 602333 Human

SwissProt: P54849 Human

Unigene: 719042 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

emp-1蛋白主要参与细胞生长发育、细胞增殖、死亡、表皮发育等作用。
产品图片
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-EMP-1 antibody (bs-0558R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
版权所有 2019-2022 体育娱乐网址平台
通过国际质量管理体系ISO9001:2008 GB/T19001-2008认证    编号:00115Q211807R1M/1100
京ICP备05066980号-1         京公网安备110107000727号