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产品编号 bs-0578R
英文名称 Collagen I
中文名称 I型胶原蛋白抗体
别    名 Collagen type I; Alpha 1 type I collagen; Alpha 2 type I collagen; COL1A1; COL1A2; Collagen I alpha 1 polypeptide; Collagen I alpha 2 polypeptide; Collagen Of Skin Tendon And Bone; Collagen Type 1; Collagen type I alpha 1; Collagen type I alpha 2; OI4; Osteogenesis Imperfecta Type IV; Pro alpha 1(I) collagen; Type I procollagen; CO1A1_HUMAN; Collagen alpha-1(II) chain; Alpha-1 type II collagen; Collagen alpha-1(II) chain; Chondrocalcin; collagen alpha-1(I) chain preproprotein; Collagen Ⅰ.  
Specific References  (42)     |     bs-0578R has been referenced in 42 publications.
[IF=1.28] Tian, Fa‑Ming, et al. "Orally administered simvastatin partially preserves lumbar vertebral bone mass but not integrity of intervertebral discs in ovariectomized rats." Experimental and Therapeutic Medicine.  IHC-P ;  Rat.  
[IF=.859] Jiang B et al. Expression of specificity protein 1 and collagen I in primary pterygial tissues. Arq Bras Oftalmol. 2019 Jun 3.   WB ;  Human.  
[IF=.68] Çakir-Özkan, Nilüfer, et al. "Therapeutic Effects of Sildenafil on Experimental Mandibular Fractures." Journal of Craniofacial Surgery 27.3 (2016): 615-620.  IHC-P ;  Rat.  
[IF=0] Yang et al. Isolation and biological characterization of tendon-derived stem cells from fetal bovine. (2016) In.Vitro.Cell.Dev.Biol.Ani. 52:846-56  IF(ICC) ;  Rat.  
[IF=0] Hendrijantini N et al. Human Umbilical Cord Mesenchymal Stem-Cell Therapy to Increase the Density of Osteoporotic Mandibular Bone. Eur J Dent. 2019 Feb;13(1):58-63.   IHC ;  Rat.  
[IF=] Luo P et al. IL-37b alleviates inflammation in the temporomandibular joint cartilage via IL-1R8 pathway. Cell Prolif. 2019 Sep 27:e12692.   IHC-P ;  Rat.  
[IF=3.024] Chen Y et al. An improved cellular enucleation method with extracellular matrix and colchicine facilitates the study of nucleocytoplasmic interaction. Eur J Cell Biol. 2019 Sep 17:151045.   ICF ;  Human.  
[IF=0] Hub Y et al. Biomimetic fabrication of Icariin loaded nano hydroxyapatite reinforced bioactive porous scaffolds for bone regeneration. Chemical Engineering Journal, 394, 124895.  IHC ;  rat.  
[IF=] Xu T et al. MicroRNA‐708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med . 2020 Jul;24(13):7127-7140.  WB ;  Human.  
[IF=] Chen Y et al. PLK1 regulates hepatic stellate cell activation and liver fibrosis through Wnt/β‐catenin signalling pathway. J Cell Mol Med . 2020 Jul;24(13):7405-7416.  WB ;  Mouse&Human.  
[IF=] Deng Z et al. Dabigatran ameliorates airway smooth muscle remodeling in asthma by modulating Yes‐associated protein. J Cell Mol Med. 2020 Jul;24(14):8179-8193.  WB&ICF ;  Mouse&Human.  
[IF=7.7] Yang, Fan, et al. "Strontium enhances osteogenic differentiation of mesenchymal stem cells and in vivo bone formation by activating Wnt/catenin signaling." Stem Cells 29.6 (2011): 981-991.  Human, Rat.  
[IF=] Zhang M et al. Hepatoprotective effect and possible mechanism of phytoestrogen calycosin on carbon tetrachloride–induced liver fibrosis in mice. Naunyn Schmiedebergs Arch Pharmacol . 2020 May 30.  WB ;  Mouse.  
[IF=] Wang Y et al. Metformin attenuates TGF‑β1‑induced pulmonary fibrosis through inhibition of transglutaminase 2 and subsequent TGF‑β pathways. 3 Biotech . 2020 Jun;10(6):287.  WB ;  Rat.  
[IF=] Wang Y et al. Metformin attenuates TGF-β1-induced pulmonary fibrosis through inhibition of transglutaminase 2 and subsequent TGF-β pathways. 3 Biotech. 2020 Jun;10(6):287.  WB ;  Rat&Human.  
[IF=] Yihang Yuet al. Human umbilical cord mesenchymal stem cell attenuates renal fibrosis via TGF-β/Smad signaling pathways in vivo and in vitro. Eur J Pharmacol . 2020 Sep 15;883:173343.  WB ;  mouse.  
[IF=] Xiajie Linet al. Leucine-activated nanohybrid biofilm for skin regeneration via improving cell affinity and neovascularization capacity. J Mater Chem B . 2020 Sep 21;8(35):7966-7976.  IHC ;  mouse.  
[IF=] Jian Songet al. 20S-Protopanaxatriol Ameliorates Hepatic Fibrosis, Potentially Involving FXR-Mediated Inflammatory Signaling Cascades. J Agric Food Chem . 2020 Aug 5;68(31):8195-8204.  WB ;  Human.  
[IF=] Min Jianget al. P2X7R orchestrates the progression of murine hepatic fibrosis by making a feedback loop from macrophage to hepatic stellate cells. Toxicol Lett . 2020 Oct 15;333:22-32.  WB, IHC ;  mouse.  
[IF=5.1] Yu, Chengyuan, et al. "Chronic obstructive sleep apnea promotes aortic remodeling in canines through miR-145/Smad3 signaling pathway." Oncotarget 8.23 (2017): 37705.  WB ;  Dog.  
[IF=4.938] Li Y et al. Klotho recovery by genistein via promoter histone acetylation and DNA demethylation mitigates renal fibrosis in mice.J Mol Med (Berl). 2019 Apr;97(4):541-552.  WB ;  Mouse&Human.  
[IF=4.45] Wang, Chen-Chie, et al. "Expandable Scaffold Improves Integration of Tissue Engineered Cartilage: An In Vivo Study in A Rabbit Model." Tissue Engineering ja (2016).  IHC-P ;  Rabbit.  
[IF=4.427] Wu M et al. Synergistic effects of sulfur dioxide and polycyclic aromatic hydrocarbons on pulmonary pro-fibrosis via mir-30c-1-3p/transforming growth factor β type II receptor axis.(2018) Chemosphere 219  WB ;  Mouse&Human.  
[IF=4.302] Ju B et al. miR-193a/b-3p relieves hepatic fibrosis and restrains proliferation and activation of hepaticstellate cells. J Cell Mol Med. 2019 Apr 3.  WB ;  Human.  
[IF=3.952] Yang KC et al. Low-adhesive ethylene vinyl alcohol-based packaging to xenogeneic islet encapsulation for type 1 diabetes treatment.Biotechnol Bioeng. 2018 Sep;115(9):2341-2355.   IHC-P ;  Rat.  
[IF=3.909] Kara A et al. Bioactive fish scale incorporated chitosan biocomposite scaffolds for bone tissue engineering. Int J Biol Macromol. 2019 Feb 20;130:266-279.   SEM, fluorescence microscopy&IHC ;  Fish(Sparus aurata).  
[IF=3.82] Luo, Yang, et al. "Alendronate Retards the Progression of Lumbar Intervertebral Disc Degeneration in Ovariectomized Rats." Bone (2013).  IHC-P ;  Rat.  
[IF=3.61] Xu, Zhen E., et al. "Inflammatory stress exacerbates lipid-mediated renal injury in ApoE/CD36/SRA triple knockout mice." American Journal of Physiology-Renal Physiology 301.4 (2011): F713-F722.  IHC-P ;  Mouse.  
[IF=3.55] Guo, Hong-Feng, et al. "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." Colloids and Surfaces B: Biointerfaces (2012).  WB ;  Mouse.  
[IF=3.42] Cecen, Berivan, et al. "Biocompatibility and biomechanical characteristics of loofah based scaffolds combined with hydroxyapatite, cellulose, poly-l-lactic acid with chondrocyte-like cells." Materials Science and Engineering: C(2016).  IHC ;  Human.  
[IF=3.405] Wu Y et al. MFAP5 promotes basal-like breast cancer progression by activating the EMT program.Cell Biosci. 2019 Mar 7;9:24.   WB ;  Human.  
[IF=3.373] Niu L et al. PLGA/PLCA casting and PLGA/PDPA electrospinning bilayer film for prevention of postoperative adhesion. (2018) J. Biomed. Mater. Res. Part B Appl. Biomater.  IHC-P ;  Rabbit.  
[IF=2.79] Yang, Ning, et al. "Caffeic acid phenethyl ester up-regulates antioxidant levels in hepatic stellate cell line T6 via an Nrf2-mediated mitogen activated protein kinases pathway." World J Gastroenterol 23.7 (2017): 1203-1214.  WB ;  Rat.  
[IF=2.16] Zhou, Nan, et al. "Involvement of Imperatorin Derivative‐OW1 in the antihypertensive effect and vascular remodeling of renovascular hypertension rats." Clinical and Experimental Pharmacology and Physiology (2014).  IHC-P ;  Rat.  
[IF=2.134] Wang et al. The influence of hypoxia during different pregnancy stages on cardiac collagen accumulation in the adult offspring. (2014) Biomed.Res.In. 2014:419805  WB ;  Rat.  
[IF=2.07] Chen, Cheng, et al. "Effects of Cytoskeleton Modulation on the Phenotype of Articular Chondrocytes in Monolayer and Pellet Culture." Journal of Biomaterials and Tissue Engineering 5.12 (2015): 942-950.  IHC-P ;  Rabbit.  
[IF=1.97] Zhang, Qiang-Ye, et al. "Administration of a novel penicillamine-bound membrane: a preventive and therapeutic treatment for abdominal adhesions." BMC surgery 11.1 (2011): 5.  Rat.  
[IF=1.93] Gao, Yuanyuan, et al. "TGF‐β1 promotes bovine mammary fibroblast proliferation through the ERK 1/2 signalling pathway." Cell biology international (2016).  IHC-P ;  Bovine.  
[IF=1.922] Chi et al. Transdermal estrogen gel and oral aspirin combination therapy improves fertility prognosis via the promotion of endometrial receptivity in moderate to severe intrauterine adhesion. (2018) Mol.Med.Rep. 17:6337-6344  IHC ;  Human.  
[IF=1.792] Huo YT et al. Methacholine induces extracellular matrix production by human airway smooth muscle cells through -catenin signaling.Respiratory Physiology & Neurobiology, 254, 55–63.   IF ;  Human.  
[IF=1.68] Ren, Xiang, et al. "Engineering zonal cartilage through bioprinting collagen type II hydrogel constructs with biomimetic chondrocyte density gradient."BMC Musculoskeletal Disorders 17.1 (2016): 1.  IHC-P ;  Rabbit.  
[IF=1.564] Liu P et al. Matrine Suppresses Pancreatic Fibrosis by Regulating TGF-β/Smad Signaling in Rats. (2019) Yonsei Med. J. 60(1)  IHC-P ;  Rat .  
研究领域 细胞生物  免疫学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Rabbit,  (predicted: Cow, Horse, Sheep, )
产品应用 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 130kDa
细胞定位 细胞外基质 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Collagen I C-terminal propeptide:1321-1400/1464 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008].

Function:
Type I collagen is a member of group I collagen (fibrillar forming collagen).

Subunit:
Trimers of one alpha 2(I) and two alpha 1(I) chains. Interacts with MRC2. Interacts with TRAM2.

Subcellular Location:
Secreted, extracellular space, extracellular matrix.

Tissue Specificity:
Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

Post-translational modifications:
Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.

DISEASE:
Defects in COL1A1 are the cause of Caffey disease (CAFFD) [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age.
Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome.
Defects in COL1A1 are the cause of Ehlers-Danlos syndrome type 7A (EDS7A) [MIM:130060]; also known as autosomal dominant Ehlers-Danlos syndrome type VII. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS7A is marked by bilateral congenital hip dislocation, hyperlaxity of the joints, and recurrent partial dislocations.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 1 (OI1) [MIM:166200]. A dominantly inherited connective tissue disorder characterized by bone fragility and blue sclerae. Osteogenesis imperfecta type 1 is non-deforming with normal height or mild short stature, and no dentinogenesis imperfecta.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 2 (OI2) [MIM:166210]; also known as osteogenesis imperfecta congenita. A connective tissue disorder characterized by bone fragility, with many perinatal fractures, severe bowing of long bones, undermineralization, and death in the perinatal period due to respiratory insufficiency.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 3 (OI3) [MIM:259420]. A connective tissue disorder characterized by progressively deforming bones, very short stature, a triangular face, severe scoliosis, grayish sclera, and dentinogenesis imperfecta.
Defects in COL1A1 are a cause of osteogenesis imperfecta type 4 (OI4) [MIM:166220]; also known as osteogenesis imperfecta with normal sclerae. A connective tissue disorder characterized by moderately short stature, mild to moderate scoliosis, grayish or white sclera and dentinogenesis imperfecta.
Genetic variations in COL1A1 are a cause of susceptibility to osteoporosis (OSTEOP) [MIM:166710]; also known as involutional or senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mass, disruption of bone microarchitecture without alteration in the composition of bone. Osteoporotic bones are more at risk of fracture.
Note=A chromosomal aberration involving COL1A1 is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGF.

Similarity:
Belongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain.
Contains 1 VWFC domain.

SWISS:
P02452

Gene ID:
1277

Database links:

·   Entrez Gene: 1277 Human

·    Entrez Gene: 12842 Mouse

·    Entrez Gene: 100008952 Rabbit

·    Entrez Gene: 29393 Rat

·    Omim: 120150 Human

·    SwissProt: P02453 Cow

·    SwissProt: O46392 Dog

·    SwissProt: P02452 Human

·    SwissProt: P11087 Mouse

·    SwissProt: P02454 Rat

·    Unigene: 172928 Human

·    Unigene: 277735 Mouse

·    Unigene: 107239 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.


I型胶原是软骨基质中的一种结构蛋白 。
主要用于Ⅰ型胶原分布及变态反应方面的研究。
产品图片
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Collagen I Polyclonal Antibody, Unconjugated(bs-0578R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Collagen I Polyclonal Antibody, Unconjugated(bs-0578R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rabbit meniscus tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Collagen I Polyclonal Antibody, Unconjugated(bs-0578R) 1:300, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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