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产品编号 bs-0649R
英文名称 CD68
中文名称 CD68抗体
别    名 CD 68; CD68 antigen; CD68 molecule; DKFZp686M18236; GP110; Macrophage antigen CD68 (microsialin); macrosialin; SCARD1; Scavenger receptor class D member 1; macrosialin isoform A precursor; CD68_MOUSE; Macrosialin; Gp110; scavenger receptor class D, member 1; macrophage antigen CD68; LAMP4.  
Specific References  (32)     |     bs-0649R has been referenced in 32 publications.
[IF=0] Stark, Christoffer, et al. "Thymosin beta 4 treatment improves left ventricular function after myocardial infarction and is related to Up-regulation of chitinase 3-like-1 in mice." Translational Medicine Communications 1.1 (2016): 8.  IHC-P ;  Mouse.  
[IF=0] Chen et al. Association of Tartrate-Resistant Acid Phosphatase-Expressed Macrophages and Metastatic Breast Cancer Progression. (2015) Medicine.(Baltimore. 94:e2165  IHC-P, IF(IHC-F) ;  Human.  
[IF=0] Nishizawa et al. IGF-I induces senescence of hepatic stellate cells and limits fibrosis in a p53-dependent manner. (2016) Sci.Re. 6:34605  IHC-P ;  Mouse.  
[IF=1.125] Nishikawa S et al. Co-Administration of Curcumin and Artepillin C Induces Development of Brown-Like Adipocytes in Association with Local Norepinephrine Production by Alternatively Activated Macrophages in Mice. J Nutr Sci Vitaminol (Tokyo). 2019;65(4):328-334.   IHF ;  Mouse.  
[IF=8.784] Wang et al. Programmed cell death-4 deficiency prevents diet-induced obesity, adipose tissue inflammation, and insulin resistance. (2013) Diabete. 62:4132-43  IHC-P ;  Mouse.  
[IF=7.78] Zhou,et al.Ultrasensitive label-free optical microfiber coupler biosensor for detection of cardiac troponin I based on interference turning point effect.(2018) Biosensors & Bioelectronics. 106:99-104.  Other ;  
[IF=] Linhe Jinet al. Tough pNAGA hydrogel hybridized porcine pericardium for the pre-mounted TAVI valve with improved anti-tearing properties and hemocompatibility. Biomed Mater . 2020 Oct 3;15(6):065013.  IHC ;  pig.  
[IF=] Yu Satoet al. Prophylactic therapy with Human amniotic fluid stem cells improved survival in a rat model of lipopolysaccharide-induced neonatal sepsis through immunomodulation via aggregates with peritoneal macrophages. Stem Cell Res Ther . 2020 Jul 20;11(1):300.  other ;  
[IF=7.126] Vendrov AE et al.NOXA1-dependent NADPH oxidase regulates redox signaling and phenotype of vascular smooth muscle cell during atherogenesis.Redox Biol. (2018) Redox Biol. 21:101063.  WB ;  Mouse.  
[IF=6.29] Müller, Irene, et al. "Pathogenic Role of the Damage-Associated Molecular Patterns S100A8 and S100A9 in Coxsackievirus B3–Induced Myocarditis." Circulation: Heart Failure 10.11 (2017): e004125.  IHC-F ;  Mouse.  
[IF=5.96] Savvatis, Konstantinos, et al. "Interleukin-6 receptor inhibition modulates the immune reaction and restores titin phosphorylation in experimental myocarditis." Basic Research in Cardiology 109.6 (2014): 1-14.  IHC-P ;  Mouse.  
[IF=5.23] Ganguly, Rituparna, et al. "Oral chromium picolinate impedes hyperglycemia-induced atherosclerosis and inhibits proatherogenic protein TSP-1 expression in STZ-induced type 1 diabetic ApoE−/− mice." Scientific Reports 7 (2017).  IHC-P ;  Mouse.  
[IF=5.11] Vendrov, Aleksandr E., et al. "Attenuated Superoxide Dismutase 2 Activity Induces Atherosclerotic Plaque Instability During Aging in Hyperlipidemic Mice." Journal of the American Heart Association 6.11 (2017): e006775.  IHC-F ;  Mouse.  
[IF=5.047] Guo G et al. Hydrogel hybrid porcine pericardium for the fabrication of pre-mounted TAVI valve with improved biocompatibility. Journal of Materials Chemistry B. 2019.  IHC-P ;  Rat.  
[IF=4.94] Xu, Xiaoyang, et al. "Simvastatin promotes NPC1‐mediated free cholesterol efflux from lysosomes through CYP7A1/LXRα signalling pathway in oxLDL‐loaded macrophages." Journal of Cellular and Molecular Medicine (2016).  IF(ICC) ;  Mouse.  
[IF=4.498] Wu et al. Chronic inflammation exacerbates glucose metabolism disorders in C57BL/6J mice fed with high-fat diet. (2013) J.Endocrino. 219:195-204  IHC-P ;  Mouse.  
[IF=4.35] Hong, Hye Kyoung, et al. "Neonatal systemic inflammation in rats alters retinal vessel development and simulates pathologic features of retinopathy of prematurity." Journal of Neuroinflammation 11.1 (2014): 87.  IHC-F ;  Rat.  
[IF=4.323] Nishikawa,et al.Highly Dispersible and Bioavailable Curcumin but not Native Curcumin Induces Brown-Like Adipocyte Formation in Mice.(2018) Molecular Nutrition & Food Research. 62:.  IF(IHC-P) ;  Mouse.  
[IF=4.2] Shang, Qianwen, et al. "Delivery of Adipose-derived Stem Cells Attenuates Adipose Tissue Inflammation and Insulin Resistance in Obese Mice through Remodeling Macrophage Phenotypes." Stem Cells and Development (2015).  IHC-P ;  Mouse.  
[IF=4.01] Xu, Xiaoyang, et al. "Lysosomal cholesterol accumulation in macrophages leading to coronary atherosclerosis in CD38−/− mice." Journal of Cellular and Molecular Medicine (2016).  IHC-F ;  Mouse.  
[IF=3.73] Zeng, Wei-qun, et al. "A New Method to Isolate and Culture Rat Kupffer Cells." PLOS ONE 8.8 (2013): e70832.  Rat.  
[IF=3.73] Chen, Liang, et al. "Electrospun Poly (L-lactide)/Poly (ε-caprolactone) Blend Nanofibrous Scaffold: Characterization and Biocompatibility with Human Adipose-Derived Stem Cells." PLOS ONE 8.8 (2013): e71265.  IHC-P ;  Rat.  
[IF=3.547] Zhang J et al. Bradykinin contributes to immune liver injury via B2R receptor-mediated pathways in trichloroethylene sensitized mice: a role in Kupffer cell activation. Toxicology. 2019 Mar 1;415:37-48.  IHC-P ;  Mouse.  
[IF=3.53] Bang, Mi-Ae, et al. "Bacillus subtilis KCTC 11782BP-Produced Alginate Oligosaccharide Effectively Suppresses Asthma via T-Helper Cell Type 2-Related Cytokines." PLoS one 10.2 (2014): e0117524-e0117524.  IHC-P ;  Mouse.  
[IF=3.44] Luo Y et al. MCC950 attenuated early brain injury by suppressing NLRP3 inflammasome after experimental SAH in rats. Brain Res Bull. 2019 Mar;146:320-326.   IF ;  Rat.  
[IF=3.33] Xiao, Ke, et al. "The role of visfatin on the regulation of inflammation and apoptosis in the spleen of LPS-treated rats." Cell and Tissue Research (2014).  WB ;  Rat.  
[IF=3.25] Kugo, Hirona, et al. "Suppressive effects of dietary EPA-rich fish oil on the degradation of elastin fibers in the aortic wall in nicotine-administered mice." Food & Function (2017).  IHC-F ;  Mouse.  
[IF=3.23] Kang, Yea Eun, et al. "The Roles of Adipokines, Proinflammatory Cytokines, and Adipose Tissue Macrophages in Obesity-Associated Insulin Resistance in Modest Obesity and Early Metabolic Dysfunction." PLOS ONE 11.4 (2016): e0154003.  IHC-P ;  Human.  
[IF=3.118] Zhao X et al. Protective effects of dioscin against systemic inflammatory response syndromevia adjusting TLR2/MyD88/NF‑κb signal pathway.(2018)Int. Immunopharmacol.Dec;65:458-469.   IF ;  Mouse&Rat.  
[IF=2.46] Wang, Shan, et al. "5-Aminolevulinic Acid–mediated Sonodynamic Therapy Reverses Macrophage and Dendritic Cell Passivity in Murine Melanoma Xenografts." Ultrasound in Medicine & Biology (2014).  IHC-P ;  Mouse.  
[IF=1.851] Bok SH et al. Allium hookeri root extract regulates asthmatic changes through immunological modulation of Th1/Th2‑related factors in an ovalbumin‑induced asthma mouse model. Mol Med Rep. 2019 Aug 6.   IHC-P ;  Mouse.  
[IF=1.396] Zhang B. TAK-242 affects Wallerian degeneration and peripheral nerve regeneration by regulating innate immunity in rats.  IHF ;  Rat.  
研究领域 免疫学  细胞表面分子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, 
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:200-800 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 37kDa
细胞定位 细胞浆 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse CD68:21-120/335 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008].

Function:
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

Subcellular Location:
Isoform Short: Cell membrane; Single-pass type I membrane protein. Isoform Long: Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein.

Tissue Specificity:
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.

Similarity:
Belongs to the LAMP family.

SWISS:
P31996

Gene ID:
12514

Database links:

Entrez Gene: 968 Human

Entrez Gene: 12514 Mouse

Omim: 153634 Human

SwissProt: P34810 Human

SwissProt: P31996 Mouse

Unigene: 647419 Human

Unigene: 15819 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CD68为巨噬细胞抗原又称酸性溶霉菌糖蛋白,表达在单核、巨嗜、粒细胞和一些梭形细胞呈阳性表达。C该抗体对于鉴定组织切片中的巨噬细胞具有重要作用。它能够鉴定多种组织中的巨噬细胞,包括脾脏红髓、肠道固有层、肺泡、骨髓中的枯否细胞和巨噬细胞。也能够识别骨髓前体细胞,外周血粒细胞以及浆细胞样T细胞。
产品图片
Sample: Bone (Mouse) Lysate at 40 ug
Primary: Anti-CD68 (bs-0649R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 37 kD
Observed band size: 76 kD
Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD68 Polyclonal Antibody, Unconjugated(bs-0649R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat myocardium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD68 Polyclonal Antibody, Unconjugated(bs-0649R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD68) Polyclonal Antibody, Unconjugated (bs-0649R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
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