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产品编号 bs-0736R
英文名称 CD56
中文名称 神经细胞粘附分子1抗体
别    名 CD56 antigen; Cell Adhesion Molecule Neural 1; MSK39; N CAM 120; N CAM 140; N CAM 180; NCAM 1; NCAM 120; NCAM 140; NCAM1; Neural cell adhesion molecule 1 120 kDa isoform; Neural cell adhesion molecule 1 140 kDa isoform; Neural cell adhesion molecule 1; NCAM1_HUMAN.  
Specific References  (2)     |     bs-0736R has been referenced in 2 publications.
[IF=5.68] Shi, Liang, et al. "Targeting Androgen Receptor (AR)→ IL12A Signal Enhances Efficacy of Sorafenib Plus NK Cells-Immunotherapy to Better Suppress HCC Progression." Molecular Cancer Therapeutics (2016): molcanther-0706.  IHC-P ;  Human.  
[IF=3.26] Liu, Xin-Qi, et al. "Regulation of neuroendocrine cells and neuron factors in the ovary by zinc oxide nanoparticles." Toxicology Letters (2016).  IHC-P ;  Chicken.  
研究领域 细胞生物  神经生物学  干细胞  细胞类型标志物  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Rat,  (predicted: Mouse, Chicken, Dog, Pig, Cow, Horse, Rabbit, )
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 92kDa
细胞定位 细胞膜 分泌型蛋白 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CD56/NCAM1:701-800/858 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]

Function:
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

Subcellular Location:
Isoform 1, 2,: Cell membrane; Single-pass type I membrane protein. Isoform 3, 4: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 5, 6: Secreted.

Similarity:
Contains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

SWISS:
P13591

Gene ID:
4684

Database links:

Entrez Gene: 4684 Human

Entrez Gene: 17967 Mouse

Entrez Gene: 24586 Rat

Omim: 116930 Human

SwissProt: P13591 Human

SwissProt: P13595 Mouse

SwissProt: P13596 Rat

Unigene: 503878 Human

Unigene: 711235 Human

Unigene: 733031 Human

Unigene: 439182 Mouse

Unigene: 4974 Mouse

Unigene: 11283 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

细胞粘附蛋白(Call Adhesion Protein)
神经元标志物

NCAM-1为神经细胞粘附分子,主要分布于神经组织,神经—肌肉接头,神经—内分泌腺和某些内分泌腺以及大多数神经外胚层来源的细胞、组织和肿瘤中。
NCAM 1主要用于视网膜母细胞瘤、髓母细胞瘤、星形细胞瘤、神经母细胞瘤等肿瘤方面的研究。CD56(神经细胞黏附分子,NCAM)表达于大部分神经外胚层来源的细胞系、组织和肿瘤如视网膜母细胞瘤、成神经管细胞瘤、星形细胞瘤以及成神经细胞瘤。它也表达于一些中胚层来源的肿瘤如横纹肌肉瘤。NK细胞以及NK细胞淋巴瘤为阳性。CD56被认为是NK细胞肿瘤的重要标志物。该抗体识别分子量为180,145和125kDa亚型。
产品图片
Protein: Hela(human)cell lysates at 40ug;
Primary: rabbit Anti-CD56 (bs-0736R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:92 kD
Observed band size:125 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NCAM1 Polyclonal Antibody, Unconjugated(bs-0736R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control:Jurkat cells(blue).
Primary Antibody:Rabbit Anti- CD56 antibody(bs-0736R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (bs-0736R,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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