扫码关注公众号           扫码咨询技术支持
客服热线:400-901-9800  客服QQ:4009019800  技术答疑  技术支持  质量反馈  人才招聘  关于我们  联系我们
体育娱乐网址平台_首页
首页 > 产品中心 > 一抗 > 产品信息
订购热线:400-901-9800
订购邮箱:sales@bioss.com.cn
订购传真:010-58129612
技术支持:techsupport@bioss.com.cn
说明书: 50ul  100ul  200ul
50ul/1098.00元
100ul/1880.00元
200ul/2900.00元
大包装/询价

产品编号 bs-0766R
英文名称 CD4
中文名称 CD4抗体
别    名 CD4 (L3T4); CD4 antigen (p55); CD4 Antigen ; CD4 molecule; CD4 Receptor; CD4+ Lymphocyte deficiency, included; CD4mut; L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; MGC165891; p55; T Cell Antigen T4 ; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T cell OKT4 deficiency, included; T cell surface antigen T4/Leu 3 ; T cell surface antigen T4/Leu3; T Cell Surface Glycoprotein CD4; W3/25; W3/25 antigen; T-cell surface glycoprotein CD4 isoform 1 precursor; CD4_MOUSE.  
Specific References  (12)     |     bs-0766R has been referenced in 12 publications.
[IF=5.345] Yuan SJ et al. Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer.J Nanobiotechnology. 2019 Oct 17;17(1):110.   IHC-P ;  Mouse.  
[IF=2.74] Lee M et al. Therapeutic DNA vaccine attenuates itching and allergic inflammation in mice with established biting midge allergy. PLoS One . 2020 Apr 23;15(4):e0232042.  IHC ;  mouse.  
[IF=] Yihan Lu  et al. Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy. Int Immunopharmacol. 2020 Aug;85:106563.  IF ;  Mouse.  
[IF=8.39] Wang, Raymond M., et al. "Humanized mouse model for assessing the human immune response to xenogeneic and allogeneic decellularized biomaterials." Biomaterials (2017).  IHC ;  Mouse.  
[IF=4.213] Wan WJ et al. Doxorubicin and siRNA-PD-L1 co-delivery with T7 modified ROS-sensitive nanoparticles for tumor chemoimmunotherapy. Int J Pharm. 2019 Jul 20;566:731-744.   IHF ;  Mouse.  
[IF=3.208] Matsubara et al. Immune activation during the implantation phase causes preeclampsia-like symptoms via the CD40-CD40 ligand pathway in pregnant mice. (2016) Hypertens.Re. 39:407-14  FCM ;  Mouse.  
[IF=3.06] Zhang, Wen, et al. "The role of CXCR3 in the induction of primary biliary cirrhosis." Clinical and Developmental Immunology 2011 (2011).  IHC-P ;  Mouse.  
[IF=3.03] Willett, Emily S., et al. "Standardized Rat Model Testing Effects of Inflammation and Grafting on Extraction Healing." Journal of Periodontology (2017): 1-15.  IHC ;  Rat.  
[IF=2.49] Ju et al. An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model. (2016) BMC.Immunol. 17:31  IHC ;  Mouse.  
[IF=2.12] Tzeng, Yuan-Sheng, et al. "Hypercapnic acidosis prolongs survival of skin allografts." Journal of Surgical Research (2014).  IHC-P ;  Mouse.  
[IF=1.85] Cheng, Lin, et al. "T cell immunohistochemistry refines lung transplant acute rejection diagnosis and grading." Diagnostic Pathology 8.1 (2013): 168.  IHC-P ;  Rat.  
[IF=1.655] Takamori S et al. HMGB1 blockade significantly improves luminal fibrous obliteration in a murine model of bronchiolitis obliterans syndrome.Takamori S et al.  IHC-P ;  Mouse.  
研究领域 细胞生物  免疫学  干细胞  细胞表面分子  淋巴细胞  t-淋巴细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, 
产品应用 WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=3μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 48kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from the middle of mouse CD4:231-330/457 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010].

Function:
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

Subunit:
Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI-CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.

Post-translational modifications:
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

Similarity:
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:
P06332

Gene ID:
12504

Database links:

Entrez Gene: 920 Human

Entrez Gene: 12504 Mouse

Omim: 186940 Human

SwissProt: P06332 Mouse

SwissProt: P01730 Human

Unigene: 631659 Human

Unigene: 2209 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

此抗体可识别55KDⅠ型单链穿膜糖蛋白。
CD4分子是存在于大多数辅助/诱导T细胞表面的59kDa的糖蛋白。正常淋巴组织中CD4的表达数量多于CD8,此抗体主要用于标记辅助/诱导T细胞,与CD8单抗联合使用对外周血淋巴细胞分型。
CD4抗原是HLA-II类分子和人类免疫缺陷病毒(HIV)-爱滋病的受体,在35-50%外周血淋巴细胞-辅助和诱导T细胞(Th/Ti)和70-80%人胸腺细胞上表达,在人的单核细胞表面也有低密度的表达。
CD4抗原有膜结合型和可溶性两种形式。Th/Ti可辅助Ig产生和T细胞毒T细胞的作用。
产品图片
Sample:
U937(Human) Cell Lysate at 30 ug
Primary: Anti- CD4 (bs-0766R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 55 kD
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-CD4 (bs-0766R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Sample:
Lymph node (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-CD4 (bs-0766R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 52 kD
Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD4) Polyclonal Antibody, Unconjugated (bs-0766R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0766R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD4 Polyclonal Antibody, Unconjugated(bs-0766R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat lung tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD4(mouse, rat) Polyclonal Antibody, Unconjugated(bs-0766R) 1:200, overnight at 4癈; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37癈. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Blank control (blue line): Mouse spleen cells(blue).
Primary Antibody (green line): Rabbit Anti-CD4/PE-CY7 Conjugated antibody (bs-0766R-PE-CY7)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-PE-CY7 .
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃ . The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
版权所有 2019-2022 体育娱乐网址平台
通过国际质量管理体系ISO9001:2008 GB/T19001-2008认证    编号:00115Q211807R1M/1100
京ICP备05066980号-1         京公网安备110107000727号