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Rabbit Anti-BrdU(Proliferation Marker) antibody (bs-0489R)
~~~促销,代码KT1102~~~
~~~促销,代码KT1101~~~

~~~20周年感恩回馈“万能卡”~~~
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说明书: 50ul  100ul  200ul
50ul/1138.00元
100ul/1980.00元
200ul/3200.00元
大包装/询价

产品编号 bs-0489R
英文名称 BrdU(Proliferation Marker)
中文名称 5-溴脱氧尿嘧啶核苷(增殖标志物)抗体
别    名 Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine.  
Specific References  (4)     |     bs-0489R has been referenced in 4 publications.
[IF=4.652] Chen et al. Sodium iodate influences the apoptosis, proliferation and differentiation potential of radial glial cells in vitro. (2014) Cell.Physiol.Bioche. 34:1109-24  IF(ICC) ;  Rat.  
[IF=3.73] Giuliani, Daniela, et al. "NDP-α-MSH induces intense neurogenesis and cognitive recovery in Alzheimer transgenic mice through activation of melanocortin MC 4 receptors." Molecular and Cellular Neuroscience (2015).  IHC-P ;  Mouse.  
[IF=3.3] Zhang et al. Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice. (2015) World.J.Gastroenterol. 21:4195-209  IHC ;  Mouse.  
[IF=2.34] Zhang, Rui, et al. "Neuroprotective Effects of Sulforaphane on Cholinergic Neurons in Mice with Alzheimer’s Disease-Like Lesions." International Journal of Molecular Sciences 15.8 (2014): 14396-14410.  IHC-P ;  Mouse.  
产品类型 药物与化合物抗体 
研究领域 细胞生物  细胞类型标志物  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, 
产品应用 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 0.3071kDa
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated Brdu: 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.
BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis

Subcellular Location:
Nuclear.

SWISS:
N/A

CAS:
59-14-3

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.


Proliferation Marker
BrdU(溴化脱氧尿嘧啶核苷)可以在体内和体外掺入到处于S期的细胞所合成的DNA链中。 此抗体可以与掺入到任何种属细胞中的BrdU反应,与碘脱氧尿 嘧啶有交叉反应,标记掺有BrdU的S期细胞,主要用于研究各种 不同的因素对正常/肿瘤组织的细胞增殖及动力学的研究。
用FITC标记的抗BrdU IgG可用于流式细胞术定量检测增值细胞。
产品图片
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Brdu Polyclonal Antibody, Unconjugated(bs-0489R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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