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Rabbit Anti-Prostaglandin E Receptor EP1/AF647 Conjugated antibody (bs-6316R-AF647)
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说 明 书: 100ul  
100ul/2980.00元
大包装/询价

产品编号 bs-6316R-AF647
英文名称 Anti-Prostaglandin E Receptor EP1/AF647
中文名称 AF647标记的前列腺素EP1受体抗体
别    名 Prostaglandin E Receptor EP1; EP1; PGE receptor EP1 subtype; PGE2 receptor EP1 subtype; Prostaglandin E receptor 1 subtype EP1 42kDa; Prostaglandin E receptor 1 subtype EP1; Prostaglandin E2 receptor EP1 subtype; Prostanoid EP1 receptor; PE2R1_HUMAN.  
规格价格 100ul/2980元 购买        大包装/询价
说 明 书 100ul  
研究领域 细胞生物  信号转导  转录调节因子  G蛋白偶联受体  G蛋白信号  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Mouse, Rat,  (predicted: Human, Dog, )
产品应用 Flow-Cyt=1ug/Test IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 42kDa
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human PTGER1/Prostaglandin E Receptor EP1
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品介绍 background:
PTGER1 is a subtype 1 receptor for prostaglandin E2 (PGE2). This receptor is coupled to the phosphatidylinositol-calcium second messenger system by G(q) proteins. PTGER1 may be an important modulator of renal function and is implicated in the smooth muscle contractile response to PGE2 in various tissues.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Abundant in kidney. Lower level expression in lung, skeletal muscle and spleen, lowest expression in testis and not detected in liver brain and heart.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

Database links:

Entrez Gene: 5731 Human

Entrez Gene: 19216 Mouse

Entrez Gene: 25637 Rat

Omim: 176802 Human

SwissProt: P34995 Human

SwissProt: P35375 Mouse

SwissProt: P70597 Rat

Unigene: 159360 Human

Unigene: 474430 Mouse

Unigene: 11423 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片
Blank control (Black line):Mouse blood(Black).
Primary Antibody (green line): Rabbit Anti-Prostaglandin E Receptor EP1 antibody (bs-6316R-AF647)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-AF647 .
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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